TABLE 1

Viability (as determined by trypan blue exclusion) and CLint,u of midazolam and propranolol in unpurified and Percoll-purified cryopreserved rat hepatocytes +/− NADPH

Data represent the mean ± S.D. (n ≥ 3).

DrugParameterUnpurifiedPercoll Purified
− NADPH+ NADPH− NADPH+ NADPH
MidazolamInitial viability (%)62 ± 386 ± 1***
Total cell concentration (106/ml)0.81 ± 0.040.58 ± 0.01***
CLint,u (µl/min per 106 cells) normalized to viable cell number262 ± 61.7399 ± 58.5§§196 ± 34.7*219 ± 33.4§§
CLint,u (µl/min per 106 cells) normalized to total cell number160 ± 31.3245 ± 31.3§§169 ± 28.4188 ± 27.5§§
PropranololInitial viability (%)67 ± 389 ± 3***
Total cell concentration (106/ml)0.37 ± 0.020.28 ± 0.01***
CLint,u (µl/min per 106 cells) normalized to viable cell number262 ± 45.4370 ± 54.2§§§182 ± 38.4162 ± 35.8§
CLint,u (µl/min per 106 cells) normalized to total cell number176 ± 26.4249 ± 33.1§§§163 ± 32.9144 ± 31.5§

  • ↵* p < 0.1,

  • ↵*** p < 0.01 using Student's paired t-test to compare unpurified and Percoll-purified preparations;

  • ↵§ p < 0.1,

  • ↵§§ p < 0.05,

  • ↵§§§ p < 0.01 using Student's paired t-test to compare CLint,u in preparations +/− NADPH.