Analytical conditions for HPLC
| Compound | Mobile Phase | Gradient Condition (B Concentration %) | |
|---|---|---|---|
| A | B | ||
| Ouabain | 5 mM ammonium formate | Acetonitrile | 1 min, 40% → 2 min, 80% → 5 min, 80% → 5.01 min, 40% → 8 min, 40% |
| Digoxin | 10 mM ammonium formate | Acetonitrile | 0.5 min, 60% → 1 min, 95% → 3 min, 95% → 3.05 min, 60% → 5 min, 60% |
| Fexofenadine | 0.1% Formic acid | Acetonitrile | 0.5 min, 18% → 1.5 min, 60% → 4 min, 60% → 4.05 min, 60% → 6 min, 18% |
| Rosuvastatin | 0.1% Formic acid | Acetonitrile | 1 min, 5% → 3 min, 45% → 3.5 min, 45% → 3.55 min, 5% → 6.5 min, 5% |
| Pravastatin | 0.1% Formic acid | Acetonitrile | 0.5 min, 25% → 1.5 min, 80% → 4 min, 80% → 4.01 min, 25% → 6 min, 25% |
| Nafcillin | 0.1% Formic acid | Acetonitrile | 1 min, 15% → 2 min, 45% → 4 min, 45% → 4.1 min, 15% → 6 min, 15% |
| Telmisartan | 10 mM ammonium acetate | Methanol | 0.5 min, 55% → 1.5 min, 80% → 4 min, 80% → 4.01 min, 25% → 6 min, 25% |