1′-Hydroxylation | 4′-Hydroxylation | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Species | Microsomes | Km | Vmax | Ki | Km | Vmax | Ki | ||||
Curcumin | Curcumin glucuronide | Curcumin sulfate | Curcumin | Curcumin glucuronide | Curcumin sulfate | ||||||
μM | nmol/min per mg protein | μM | μM | μM | nmol/min per mg protein | μM | μM | μM | |||
Cynomolgus monkey | Liver | 8.88 | 8.28 | 8.37 | — | 17.0 | 53.7 | 9.99 | 11.9 | — | 19.5 |
Small intestine | 1.49 | 1.96 | 1.71 | — | 46.6 | 6.45 | 0.577 | 2.56 | — | 14.3 | |
Human | Liver | 3.94 | 4.86 | 3.45 | — | 3.20 | 26.9 | 1.82 | 6.45 | — | 6.95 |
Small intestine | 2.27 | 0.449 | 1.43 | — | 4.54 | 12.8 | 0.0968 | 3.14 | — | 7.26 |
Ki, the inhibition constant; Km, the velocity, the Michaelis-Menten constant; Vmax, the maximum enzyme velocity without inhibitors.