TABLE 1

Summary of cross-linking reaction of single and double mutants of 3Cys ΔMRP1

Numbers correspond to the thiosulfonate reagent that cross-linked cysteine residues in a given mutant. For instance, “5” corresponds to M5M. Dash (–) indicates no cross-linked product detected with any of the three cross-linkers. Empty cells indicate that the cross-linking reactions were not performed.

TM8TM16TM15TM14
MutantR433CY1190CI1193CE1144CS1145CD1081CT1082C
3Cys ΔMRP15, 8, 1717
R433C 3Cys ΔMRP15, 8, 178, 1717