Oligonucleotides used in this study
| Forward (5′ to 3′) | Reverse (5′ to 3′) | |
|---|---|---|
| qRT-PCR | ||
| hLRH-1 | CTTTGTCCCGTGTGTGGAGAT | GTCGGCCCTTACAGCTTCTA |
| hOAT2 | AGCCTCCGTCAGCTATGTAAT | CATCGCCAGTCCCGTATCA |
| hGAPDH | CATGAGAAGTATGACAACAGCCT | AGTCCTTCCACGATACCAAAGT |
| mLrh-1 | GAACTGTCCAAAACCAAAAAAGG | CTTCCAGCTTCATCCCAAC |
| mOat2 | CAACTGCGGAATCTGGTGCT | ATCAGGCAGGGCACAATGATG |
| mOatp1b2 | CCTGAGAAGTGTCCGCATAAC | GTCCGAGTGGCAGTAAGAAAG |
| mOatp1a1 | GTGCATACCTAGCCAAATCACT | CCAGGCCCATAACCACACATC |
| mOatp1a4 | GCTTTTCCAAGATCAAGGCATTT | CGTGGGGATACCGAATTGTCT |
| mCyp8b1 | GCCTTCAAGTATGATCGGTTCCT | GATCTTCTTGCCCGACTTGTAGA |
| mPpib | TCCACACCCTTTTCCGGTCC | CAAAAGGAAGACGACGGAGC |
| Reporter plasmid | ||
| hOAT2(−1100/+28) | CGCTTTCTCAGGGATCCCAGCCTGGAG | CCTCAAAGCCCATGCTGCTCACCCA |
| hOAT2(−300/+28) | GGCCCCAGGCCCCAGGGGCCTCCTCTT | CCTCAAAGCCCATGCTGCTCACCCA |
| hOAT2(−100/+28) | AGACAGGTAGAGGTCCCTGGAGAC | CCTCAAAGCCCATGCTGCTCACCCA |
| mOat2(−1100/+89) | CCGCTCGAGAATTTTCTCAGAAATGCAAA | CCCAAGCTTAGCCACCCACCTTGTGCAGCA |
| mOat2(−400/+89) | CCGCTCGAGTGAGTCAAACTGTGCTGTGCTT | CCCAAGCTTAGCCACCCACCTTGTGCAGCA |
| mOat2(−150/+89) | CCGCTCGAGGGAATGGTGGCCTTTCCCCTTAG | CCCAAGCTTAGCCACCCACCTTGTGCAGCA |
| EMSA | ||
| Cyp7a1 | GGACTTAGTTCAAGGCCAGATAATGCT | AGCATTATCTGGCCTTGAACTAAGTCC |
| Oat2 | TCTTCCGAAGGCCCTGAGTTCCATTCC | GGAATGGAACTCAGGGCCTTCGGAAGA |
| Oat2(mutant) | TCTTCCACCTGTATGCGACCGCATTCC | GGAATGCGGTCGCATACAGGTGGAAGA |
| CHIP | ||
| Cyp7a1 | GCTTATCGACTATTGCAGCTCTCT | CTGGCCTTGAACTAAGTCCATCT |
| Oat2 | AAAGAGGCAGACTGGACT | GTTGCTGGGAATGGAACT |
| siRNA | ||
| siLrh-1-1 | GCAUGGACUUACAGCUAUUTT | AAUAGCUGUAAGUCCAUGCTT |
| siLrh-1-2 | CCUUGUCAUGCUGCCCAAATT | UUUGGGCAGCAUGACAAGGTT |
| siLrh-1-3 | GCAGAAGAAAGCCCUCAUUTT | AAUGAGGGCUUUCUUCUGCTT |
| siLrh-1-4 | CCAACCCUAUGGUCACUUUTT | AAAGUGACCAUAGGGUUGGTT |
| siLRH-1 | AAGGATCCATCTTCCTGGTTA | TAACCAGGAAGATGGATCCTT |