TABLE 2

Reaction parameters derived from the cytotoxicity and membrane vesicular transport assays

Assay	IC₅₀ (µM)^{^a}
Cytotoxicity	Calcitriol	Calcipotriol
H69	29.0 ± 1.2^*	23.9 ± 0.5^*
H69AR (control)	11.0 ± 0.7	8.5 ± 1.0
H69AR + 10 μM MK571	16.4 ± 1.9	15.0 ± 1.1^*
H69AR + 50 μM MK571	61.6 ± 1.2^**	42.4 ± 1.0^**
HEK293/pcDNA3.1	33.4 ± 2.5^**	23.3 ± 3.7
HEK293/MRP1 (control)	8.9 ± 1.3	6.0 ± 1.8
HEK293/MRP1+ 10 μM MK571	11.7 ± 1.1	16.5 ± 0.4^*
HEK293/MRP1+ 50 μM MK571	35.3 ± 4.0^**	27.5 ± 0.2^**
HEK293/P-gp	43.1 ± 4.3^**	37.9 ± 5.1^**
HEK293/BCRP	31.3 ± 5.3^**	20.8 ± 2.7^*
Membrane vesicular transport
E₂17βG	9.0 ± 1.3	12.3 ± 0.1

↵^a Mean ± S.E.M. of n = 3 independent experiments.
↵* P < 0.05; **P < 0.01 significantly different then the control in each cell line group, calculated using linear mixed model with Sidak post hoc test.