TABLE 3

Effect of calcitriol and calcipotriol (10 µM) on IC50 of vincristine and mitoxantrone in HEK293/P-gp and HEK293/BCRP, respectively

Verapamil (25 µM) and Ko143 (1 µM) are used as positive controls.

Cell line/TreatmentVincristine, IC50 (nM)aMitoxantrone, IC50 (nM)a
HEK293/pcDNA3.13.70 ± 1.26***4.57 ± 1.48***
HEK293/P-gp (control)1394.00 ± 174.46
HEK293/P-gp + verapamil2.95 ± 1.16***
HEK293/P-gp + calcitriol758.93 ± 323.48*
HEK293/P-gp + calcipotriol982.60 ± 118.54
HEK293/BCRP (control)30.88 ± 4.18
HEK293/BCRP + Ko1431.33 ± 0.14***
HEK293/BCRP + calcitriol12.84 ± 0.26**
HEK293/BCRP + calcipotrioll29.66 ± 3.21

  • ↵a Mean ± S.E.M. of n = 3 independent experiments.

  • ↵* P < 0.05; **P < 0.01; ***P < 0.001 significantly different then control in each treatment group, calculated using linear mixed model with Sidak post hoc test.