TABLE 1

The mean values and S.E. of kinetic parameters of resorufin formation by human recombinant CYP1B1 in the absence and presence of 19(R)-HETE or 19(S)-HETE

Results are presented as mean and S.E., of at least three individual experiments. Km, Vmax and Ki mean ± S.E. were determined by the Enzyme Kinetics module of GraphPad Prism, version 5.01.

KmVmaxKi
19(R)-HETE19(S)-HETE
nMpmol/pmol P450 per minutenMnM
86.6 ± 10.416.7 ± 1.189.1 ± 11.537.3 ± 5.5