TABLE 1

Screening gut bacteria for tacrolimus conversion to M1 in YCFA culture

OrderBacteriumOD600M1 Production Detected
BifidobacterialesBifidobacterium longum1.8No
BacteroidalesBacteroides cellulosilyticus0.6Yesa
Bacteroides finegoldii3.4Yesa
Bacteroides ovatus4.2Yesa
Parabacteroides merdae2.7Yesa
Parabacteroides johnsonii3.6Yesa
Parabacteroides goldsteinii3.3Yesa
ClostridialesRuminococcaceae sp.0.5Yes
Clostridium innocuum3.4Yes
Anaerostipes sp.2.7Yes
Dorea formicigenerans2.4Yes
Clostridium clostridioforme3.0Yes
Clostridium hathewayi2.6Yes
Blautia sp.4.7Yes
Clostridium aldenense1.4Yes
Clostridium symbiosum2.5Yes
Clostridium citroniae1.7Yes
Coprococcus sp.2.4Yes
Clostridium bolteae3.6Yes
Clostridium cadaveris1.4Yes
Ruminococcus gnavus3.4Yes
ErysipelotrichalesErysipelotrichaceae sp.3.8Yes
  • a M1 production observed only when using sensitive HPLC-MS/MS for detection.