Injection protocols for cocktail probes
Day 0 indicates these groups received only a single injection of DTX injection or PEG-DTX-L. Day 3 indicates these groups received repeated injections of DTX or PEG-L with a 3-day interval.
| Group | First Injection | Time Interval | Second Injection (2.5 mg/kg) + Cocktail Probes (1.0 mg/kg) |
|---|---|---|---|
| day | |||
| Control | PBS | 3 | PBS + cocktail probes |
| Single DTX injection | DTX injection (2.5 mg/kg) | 0 | Cocktail probes |
| Repeated DTX injection | DTX injection (2.5 mg/kg) | 3 | DTX injection + cocktail probes |
| Single PEG-L injection | PEG-DTX-L (2.5 mg/kg) | 0 | Cocktail probes |
| Repeated PEG-L injection | PEG-B-L | 3 | PEG-DTX-L + cocktail probes |