cDNA | Vmax ± S.D. × 10−3 | Km ± S.D. | CLINT × 10−4 (%WT) | Polyphen | SIFT | PROVEAN | Enzyme Activity in Relation to WT | Concordance Yes/No | |
---|---|---|---|---|---|---|---|---|---|
Vivid* | MS* | ||||||||
nmol/mg per minute | µM | µl/mg protein per minute | % | % | |||||
WT | 67.2 ± 2.51 | 30.1 ± 7.79 | 23.4 ± 6.27 | ||||||
65A>G | 33.3 ± 0.74 | 40.3 ± 0.86 | 8.25 ± 0.36 (36.2) | Benign | Tolerated | Deleterious | 49 | 36 | No |
337G>A | 23.6 ± 2.75 | 96.6 ± 23.1 | 2.49 ± 0.28 (10.7)* | Benign | Tolerated | Neutral | 75 | 11 | No |
518C>T | 3.65 ± 0.25 | 205 ± 11.8 | 0.18 ± 0.02 (0.7)* | Probably Damaging | Tolerated | Deleterious | 5 | 1 | Yes |
556C>T | 3.03 ± 0.36 | 148 ± 69.5 | 0.23 ± 0.09 (1.6)* | Probably Damaging | Damaging | Deleterious | 7 | 2 | Yes |
557G>A | 4.33 ± 0.34 | 30.5 ± 7.12 | 1.45 ± 0.20 (6.5)* | Probably Damaging | Tolerated | Deleterious | 7 | 7 | Yes |
578A>G | 68.5 ± 4.69 | 51.5 ± 7.69 | 13.4 ± 1.06 (57.9) | Benign | Tolerated | Neutral | 106 | 58 | Yes |
815A>G | 35.2 ± 0.11 | 38.4 ± 2.47 | 9.19 ± 0.54 (39.7) | Benign | Damaging | Deleterious | 53 | 40 | Yes |
*3 | 0.02 ± 0.01 | 4.82 ± 0.00 | 0.05 ± 0.02 (0.2)* | 3 | N/A |
CLINT, intrinsic clearance; Km, Michaelis constant; MS, mass spectrometry; N/A, not applicable.
↵* P < 0.05 compared with CYP2C19 WT.