TABLE 3

Final region-specific abundances along the gastrointestinal tract normalized to jejunum I based on relative abundance quantification or by combining abundance data obtained from relative and absolute quantification methodology

Protein	ADAM Model Segment (Sample Number Quantified Is Given in Parentheses)								Reference
Protein	Duodenum	Jejunum I^{^a}	Jejunum II	Ileum I	Ileum II	Ileum III	Ileum IV	Colon	Reference
ABCB1 (P-gp)^{^b}^,^{^c}	0.51 (31)	1 (9)	1.46 (8)	1.50 (42)	1.51 (42)	1.52 (42)	1.51 (42)	0.57 (27)	See Table 2 in Harwood et al. (2013) for references
ABCC1 (MRP1)^{^b}	0.45 (37)	1 (9)	0.88 (9)	0.86 (37)	0.86 (37)	0.89 (37)	0.89 (37)	0.93 (50)	Fromm et al. (2000), Albermann et al. (2005), Zimmermann et al. (2005), Berggren et al. (2007), Blokzijl et al. (2007), Bourgine et al. (2012), Drozdzik et al. (2014)
ABCC2 (MRP2)^{^b}^,^{^c}	1.41 (71)	1 (4)	1 (4)	0.60 (41)	0.60 (41)	0.60 (41)	0.60 (41)	0.02 (26)	See Table 2 in Harwood et al. (2013) for references
ABCC3 (MRP3)	2.15 (29)	1 (16)	0.89 (16)	1.54 (35)	1.54 (35)	1.60 (35)	1.60 (35)	5.95 (35)	Zimmermann et al. (2005), Englund et al. (2006), Seithel et al. (2006), Bourgine et al. (2012), Gröer et al. (2013), Drozdzik et al. (2014)
ABCC4 (MRP4)^{^b}	1.02 (15)	1 (6)	1.22 (6)	1.71 (19)	1.71 (19)	1.20 (19)	1.20 (19)	1.76 (19)	Zimmermann et al. (2005), Bourgine et al. (2012), Drozdzik et al. (2014)
ABCG2 (BCRP)^{^b}^,^{^c}	0.47 (45)	1 (14)	1 (45)	0.59 (45)	0.59 (45)	0.59 (45)	0.59 (45)	0.13 (35)	See Table 2 in Harwood et al. (2013) for references
SLC2A2 (GLUT2)^{^b}	1 (15)	1 (0)^{^d}	1 (0)^{^d}	1 (0)^{^d}	1 (0)^{^d}	1 (0)^{^d}	1 (0)^{^d}	1 (0)^{^d}	Wilder-Smith et al. (2014)
SLC10A2 (ASBT/IBAT)	16.49 (30)	1 (6)	4 (6)	98.44 (59)	98.44 (59)	109.18 (59)	108.00 (59)	1.10 (56)	Hruz et al. (2006), Meier et al. (2007), Wojtal et al. (2009), Bourgine et al. (2012), Gröer et al. (2013), Drozdzik et al. (2014)
SLC15A1 (PEPT1)	0.94 (35)	1 (20)	1.06 (20)	1.23 (67)	1.23 (67)	1.24 (67)	1.24 (67)	0.03 (63)	Ziegler et al. (2002), Englund et al. (2006), Seithel et al. (2006), Meier et al. (2007) Wojtal et al. (2009), Bourgine et al. (2012), Gröer et al. (2013), Oswald et al. (2013), Drozdzik et al. (2014)
SLC16A1 (MCT1)^{^b}	1.25 (17)	1 (13)	1 (13)	1.29 (20)	1.29 (20)	1.29 (20)	1.29 (20)	4.72 (26)	Gill et al. (2005), Englund et al. (2006), Seithel et al. (2006), Bourgine et al. (2012)
SLCO2B1 (OATP2B1)	0.73 (30)	1 (20)	0.94 (20)	1.28 (76)	1.28 (76)	1.28 (76)	1.28 (76)	1.06 (58)	Englund et al. (2006), Seithel et al. (2006), Meier et al. (2007), Wojtal et al. (2009), Bourgine et al. (2012), Gröer et al. (2013), Oswald et al. (2013), Drozdzik et al. (2014)
SLCO4C1 (OATP4C1)^{^b}	1 (0)^{^d}	1 (0)^{^d}	1 (0)^{^d}	1 (3)^{^e}	1 (3)^{^e}	1 (3)^{^e}	1 (3)^{^e}	1 (3)^{^e}	Bourgine et al. (2012)
SLC22A1 (OCT1)	1.03 (6)	1 (6)	0.87 (6)	1.29 (35)	1.29 (35)	1.30 (35)	1.30 (35)	2.77 (32)	Wojtal et al. (2009), Bourgine et al. (2012), Gröer et al. (2013), Drozdzik et al. (2014)
SLC22A3 (OCT3)	1.19 (6)	1 (6)	1.11 (6)	1.08 (12)	1.08 (12)	1.23 (12)	1.23 (12)	1.88 (9)	Bourgine et al. (2012), Gröer et al. (2013), Drozdzik et al. (2014)
SLC22A4 (OCTN1)^{^b}	0.46 (16)	1 (6)	0.63 (6)	0.78 (51)	0.78 (51)	0.80 (51)	0.80 (51)	0.24 (49)	Meier et al. (2007), Wojtal et al. (2009), Bourgine et al. (2012), Girardin et al. (2012), Drozdzik et al. (2014)
SLC51A/B (OST-α/β)^{^f}	0.56 (6)	1 (6)	1.89 (7)	1.08 (40)	1.08 (40)	0.93 (40)	0.93 (41)	0.71 (6)	Renner et al. (2008), Drozdzik et al. (2014), Harwood (2015)
SLC51B (OST-β)^{^f}	0.6 (6)	1.19 (6)	1.16 (9)	1.01 (40)	1.01 (40)	1 (40)	1 (41)	0.33 (6)	Renner et al. (2008), Drozdzik et al. (2014), Harwood (2015)

ASBT, apical sodium-dependent bile acid transporter; BCRP, breast cancer resistance protein; MCT1, monocarboxylate transporter 1; PEPT1, peptide transporter 1.
↵^a Fixed value of 1: jejunum I was fixed to 1 as the final value; however, prior to normalization a weighted mean relative expression was calculated across samples with the potential for CV generation, hence the sample number provided.
↵^b Where only relative abundance data were available.
↵^c Final values not updated from those previously reported (Harwood et al., 2013) since the region-specific absolute abundance values in Supplemental Table 3 were similar.
↵^d Where samples were not quantified within a given region a relative abundance value of 1 was assumed.
↵^e Since there were no jejunum I values available to normalize the ileum and colon abundances to the relative expression for these segments, the value was set to 1.
↵^f Used OST-α values from relative and absolute combined analysis—for completeness the OST-β segmental relative abundance values are given in the last row of the table.