TABLE 1

Primers used in qRT-PCR analysis

NamePrimer Sequence (5′–3′)
Mouse-FXR-FTCC​GGA​CAT​TCA​ACC​ATC​AC
Mouse-FXR-RTCA​CTG​CAC​ATC​CCA​GAT​CTC
Mouse-FGF15-FGAG​GAC​CAA​AAC​GAA​CGA​AAT​T
Mouse-FGF15-RACG​TCC​TTG​ATG​GCA​ATC​G
Mouse-SHP-FCGA​TCC​TCT​TCA​ACC​CAG​ATG
Mouse-SHP-RAGG​GCT​CCA​AGA​CTT​CAC​ACA
Mouse-OSTα-FGCC​AGG​CAG​GAC​TCA​TAT​CAA​A
Mouse-OSTα-RGGC​AAC​TGA​GCC​AGT​GGT​AAG​A
Mouse-FGFR4-FCAG​GTC​TGC​CAA​ATC​CTT​GT
Mouse-FGFR4-RCAG​AGG​CCT​TTG​GTA​TGG​AT
Mouse-CYP7A1-FAGC​AAC​TAA​ACA​ACC​TGC​CAG​TAC​TA
Mouse-CYP7A1-RGTC​CGG​ATA​TTC​AAG​GAT​GCA
Mouse-BSEP-FCAC​ACA​AAG​CCC​CTA​CCA​GT
Mouse-BSEP-RCCA​GAG​GCA​GCT​ATC​AGG​AC
Mouse-LDLR-FAGG​CTG​TGG​GCT​CCA​TAG​G
Mouse-LDLR-RTGCGGTCCAGGGTCATCT
Mouse-G6Pase-FGTG​CAG​CTG​AAC​GTC​TGT​CTG​TC
Mouse-G6Pase-RTCC​GGA​GGC​TGG​CAT​TGT​A
Mouse-PEPCK-FGTG​TTT​GTA​GGA​GCA​GCC​ATG​AGA
Mouse-PEPCK-RGCC​AGT​GGG​CCA​GGT​ATT​TG
Mouse-β-actin-FATG​GAG​GGG​AAT​ACA​GCC​C
Mouse-β-actin-RTTC​TTT​GCA​GCT​CCT​TCG​TT
Human-FXR-FTGC​ATT​GAA​GTT​GCT​CTC​AGG​T
Human-FXR-RCGC​CTG​ACT​GAA​TTA​CGG​ACA
Human-FGF19-FCAC​GGG​CTC​TCC​AGC​TGC​TTC​CTG​CG
Human-FGF19-RTCC​TCC​TCG​AAA​GCA​CAG​TCT​TCC​TCC​G
Human-β-actin-FGCG​TGA​CAT​TAA​GGA​GAA​G
Human-β-actin-RGAAGGAAGGCTGGAAGAG

  • BSEP, bile salt export pump; F, forward; qRT-PCR, quantitative real-time PCR; R, reverse.