Regular ArticlecDNA Cloning and Characterization of a Novel Member of Steroid-Induced Cytochrome P450 3A in Rats
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Species differences in in vitro and in vivo small intestinal metabolism of CYP3A substrates
2008, Journal of Pharmaceutical SciencesCitation Excerpt :The expression of CYP1A1, 2B1, 2C6, 2C11, 2D, and 3A1 mRNA were detectable in rat small intestine, and further, expression of CYP1A1, 2B1, and 3A1 protein were confirmed by immunoblot analysis using antiserum against CYP1A1/2, CYP3A1/2, and CYP2B1/2. To date, six CYP3A enzymes expressed in the rat have been reported as follows: CYP3A1,53 CYP3A2,54 CYP3A9,55 CYP3A18,56, 57 CYP3A23,58, 59 and CYP3A62.60 Surprisingly, an expression profile of CYP3A in rat small intestine was shown by Takara et al.,61 with no detection of PCR products for CYP3A1 and 3A2, but PCR products were found for CYP3A9 and 3A18.
Safety Evaluation and Drug Development based on Biological Fate of Drugs Efforts Made to Overcome Drug Interaction in Drug Developments
2002, Drug Metabolism and PharmacokineticsIdentification and characterization of a cDNA encoding cytochrome P450 3A from the fresh water teleost medaka (Oryzias latipes)
2000, Archives of Biochemistry and BiophysicsInduction of rat hepatic drug metabolizing enzymes by dimethylcyclosiloxanes
2000, Chemico-Biological InteractionsCitation Excerpt :The CYP3A subfamily is important in the metabolism of a variety of clinically and toxicologically important compounds including steroids, barbiturates, and macrolide antibiotics [12]. At present, five CYP3A genes have been detected in rat liver: CYP3A1 [19], CYP3A2 [20], CYP3A9 [21], CYP3A18 [22], and CYP3A23 [23]. CYP3A23 is closely related to CYP3A1 and may not be easily differentiated by either immunochemical analysis or activity assay.
Methodologies to study the induction of rat hepatic and intestinal cytochrome P450 3A at the mRNA, protein, and catalytic activity level
2000, Journal of Pharmacological and Toxicological Methods