Regular ArticleActive Site Mutations of Cytochrome P450cam Alter the Binding, Coupling, and Oxidation of the Foreign Substrates (R)- and (S)-2-Ethylhexanol
References (31)
- et al.
J. Biotechnol.
(2001) - et al.
Biochim. Biophys. Acta
(2001) - et al.
Biochem. Biophys. Res. Commun.
(1992) - et al.
J. Biol. Chem.
(1984) - et al.
Methods Enzymol.
(1978) - et al.
J. Mol. Biol.
(1987) - et al.
J. Biol. Chem.
(1982) Curr. Opin. Struct. Biol.
(1995)- et al.
Biochem. Biophys. Res. Commun.
(1978) - et al.
J. Biol. Chem.
(1995)
J. Biol. Chem.
J. Biol. Chem.
Arch. Biochem. Biophys.
J. Biotechnol.
Protein Eng.
Cited by (31)
Fate of di (2-ethylhexyl) phthalate and its impact on soil bacterial community under aerobic and anaerobic conditions
2019, ChemosphereCitation Excerpt :As observed in the previous study by Zhu et al. (2018a), the monoester MEHP was found to be a major degradation product (Fig. 2c and d). It was somewhat surprising to find that both aerobic and anaerobic soils also contained high amounts of 2-ethylhexanol (241–601 μg kg−1) (Fig. 2c and d), considering that it can be readily bio-transformed or degraded in previous studies (French et al., 2002; Wyatt et al., 1987). Indeed, significant amounts of 2-ethylhexanol have also been reported during DEHP degradation by pure cultures (Nalli et al., 2003) or by complex microbial community in sediments (Horn et al., 2004).
In vivo and in vitro hydroxylation of cineole and camphor by cytochromes P450CYP101A1, CYP101B1 and N242A CYP176A1
2016, Journal of Molecular Catalysis B: EnzymaticCitation Excerpt :Structural investigations including both the crystallographic [10–16] and in silico studies [17–19] have established that a variety of different molecules can either bind or are predicted to bind within the active site of P450cam. Further investigations have demonstrated that P450cam is also able to catalyse the oxidation of some of these molecules to chemically useful compounds, which has consequently led to development of mutants that are optimised to catalyse the oxidation of alternative substrates [20–33]. For example, a number of active-site mutations introduced into P450cam were found to improve its selectivity toward two monoterpenes: S-limonene and (+)-α-pinene [22,24,34].
Cytochromes P450 as versatile biocatalysts
2006, Journal of BiotechnologyHeteroactivator effects on the coupling and spin state equilibrium of CYP2C9
2006, Archives of Biochemistry and BiophysicsPlasticizer metabolites in the environment
2004, Water ResearchCitation Excerpt :This is important because the alcohol has a higher acute toxicity than the acid (Table 1). This was unexpected because biological systems have been shown to be able to easily oxidize alcohols including 2-ethylhexanol to the corresponding carboxylic acids (Cheung et al., 2003; French et al., 2002). River sediments had the highest concentrations of the alcohol and acid.
- 1
Current address: Department of Pharmacology, Pennsylvania State University College of Medicine, Hershey, PA 17033.
- 2
To whom correspondence should be addressed at Department of Chemistry, 477 Fulmer Synthesis, Washington State University, Pullman, WA 99164-4630. Fax: (509) 335-8867.