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cDNA Cloning and Functional Expression of a Novel Rat Kidney Organic Cation Transporter, OCT2

https://doi.org/10.1006/bbrc.1996.1056Get rights and content

Abstract

A cDNA encoding a novel organic cation transporter, designated as OCT2 was isolated from the rat kidney. The rat OCT2 cDNA (2,205 bp) had an open reading frame encoding for a 593-amino acid protein (calculated molecular mass of 66 kDa) which shows 67 % identity with the rat OCT1. Northern hybridization and reverse transcription-PCR analyses revealed that the rat OCT2 mRNA transcript was expressed predominantly in the kidney, at higher level in the medulla than in the cortex, but this transcript was not detected in the brain, heart, lung, liver, small intestine or spleen. When expressed inXenopusoocytes, rat OCT2 stimulated the uptake of tetraethylammonium, and the uptake was markedly inhibited by the presence of cimetidine, procainamide and quinidine. These findings suggest that OCT2 is responsible for the transport of cationic drugs in the kidney.

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    Citation Excerpt :

    Besides, low expression of human OCT1 is also detected in other tissues including small intestine, colon, kidney, lung, brain, heart, skeletal muscle, peripheral leukocytes, adrenal gland, mammary gland, immune cells and adipose tissue [29,32,33]. Oct2 was isolated by homology screening from rat kidney and human OCT2 was also cloned later [32,34]. In humans, OCT2 is predominantly expressed on the basolateral membrane of renal proximal tubule cells in the kidney, and low expression has also been reported in brain, lung, small intestine, thymus, placenta and the inner ear [29,32].

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Abbreviations: RT, reverse transcription; PCR, polymerase chain reaction.

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