Regular Article
Purification and Characterization of Rabbit Small Intestinal Cytochromes P450 Belonging to CYP2J and CYP4A Subfamilies

https://doi.org/10.1006/bbrc.1997.6342Get rights and content

Abstract

A new form of P450 designated P450ib2 was purified from rabbit small intestine microsomes. This P450 had properties very similar to P450ib (CYP2J1), and showed 88% identity with CYP2J1 in its first 20 NH2-terminal amino acid sequence, excluding 3 undetermined residues. Both P450ib and P450ib2 were immunohistochmically detected in the mucosal epitherial cells of the duodenum, jejunum, and ileum in the small intestine, whereas no immunoreactivity was observed in other tissues including liver, kidney, lung, colon, and stomach. The results support that the two closely related P450s are specifically localized in the rabbit small intestine. Another small intestinal P450, P450ia, was found to hydroxylate a wide variety of fatty acids including straight-chain, branched-chain, unsaturated, or hydroxy fatty acids, and prostaglandin A at the ω and (ω-1) positions. P450ia was identical with a rabbit kidney fatty acid ω-hydroxylase, CYP4A7, in its 25 NH2-terminal amino acid sequence, excluding 2 undetermined residues. The results identify P450ia as CYP4A7.

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    Abbreviations: P450, cytochrome P450CYP, cytochrome P450 (Ref. 1);

    1

    To whom correspondence should be addressed. Fax: +81 849 36 2023.

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