Regular ArticleNucleotide Binding and Sulfation Catalyzed by Phenol Sulfotransferase☆
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2005, Biochemical and Biophysical Research CommunicationsCitation Excerpt :Miscellaneous methods. PAP[35S] was synthesized from ATP and carrier-free [35S]sulfate using the bifunctional human ATP sulfurylase/adenosine 5′-phosphosulfate kinase and its purity was determined as previously described [18]. The PAP[35S] synthesized was adjusted to the required concentration and specific activity of 15 Ci/mmol at 1.4 mM by the addition of cold PAPS.
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Abbreviations used: PAPS, 3′-phospho adenosine 5′-phosphosulfate; PAP, 3′-phospho adenosine 5′-phosphate or adenosine 3′, 5′-bisphosphate; 2′, 5′-PAP, adenosine 2′, 5′-bisphosphate; 2′:3′-cyclic PAP, adenosine 2′:3′-cyclic phosphate 5′-phosphate; AMP, adenosine 5′-monophosphate; ADP, adenosine 5′-diphosphate; pNPS, 4-nitrophenyl sulfate; pNP, 4-nitrophenol; PST, phenol sulfotransferase.
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To whom correspondence should be addressed at Department of Biological Science and Technology, National Chiao Tung University, 75 Po-Ai Street, Hsinchu, Taiwan, ROC. Fax: 886-3-5729288. E-mail: [email protected].