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Full-Length cDNA Cloning and Genomic Organization of the Mouse Liver-Specific Organic Anion Transporter-1 (lst-1)

https://doi.org/10.1006/bbrc.2000.2830Get rights and content

Abstract

We have cloned a cDNA that codes for mouse liver-specific transporter-1, mouse lst-1. The cDNA is comprised of 3296 base pairs and it contains a coding sequence for a protein of 689 amino acids with 12 putative transmembrane domains. The deduced amino acid sequence of the mouse lst-1 shares 64 and 77% identities with the reported human and rat lsts, respectively. Northern blot analysis demonstrates that mouse lst-1 mRNA is expressed exclusively in liver. We also report here the structural organization of the mouse lst-1 gene as the first evidence for the structure of a gene encoding an lst. The mouse lst-1 gene spans approximately 60 kbp in length and consists of 16 exons, including two noncoding exons. All the introns are flanked by GT-AG consensus splice sequences. 5′-Rapid Amplification of cDNA Ends (RACE) analyses demonstrate three splice variant mRNAs involving the noncoding exon 2 and exon 3. The 5′-flanking region of the gene contains consensus CAAT and TATA boxes and several potential binding sites for transcription factors for CAAT enhancer binding protein (C/EBP) and hepatocyte nuclear factors (HNF-3β, HFH-1, and HFH-2), transcription factors important for liver-specific gene expression.

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    Sequence data from this article have been deposited in DDBJ/EMBL/GenBank data bases under the Accession Nos. AB031959 and AB037190 through AB037202.

    1

    Present address: Department of Drug Metabolism and Molecular Toxicology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.

    2

    To whom correspondence should be addressed: Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, 3901 Rainbow Blvd., Kansas City, KS 66160-7417. Fax: (913) 588-7501. E-mail: [email protected].

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