Biochemical Characterization and Tissue Distribution of Human SULT2B1

doi:10.1006/bbrc.2001.5746

Biochemical and Biophysical Research Communications

Volume 288, Issue 1, 19 October 2001, Pages 280-289

https://doi.org/10.1006/bbrc.2001.5746 Get rights and content

Abstract

The human hydroxysteroid sulfotransferase (SULT) family is comprised of two subfamilies, SULT2A1 and SULT2B1. We characterized the substrate specificity, in vitro biochemical properties, and tissue distribution patterns of human SULT2B1a and SULT2B1b. In contrast to the wide substrate specificity of SULT2A1, SULT2B1a and SULT2B1b specifically catalyzed the sulfonation of 3β-hydroxysteroids with high catalytic efficiency. Both SULT2B1 enzymes also sulfonated dihydrotestosterone. In vitro studies revealed that the biochemical properties of SULT2B1a and SULT2B1b were not significantly different from each other. However, tissue expression analysis suggested that they are differentially regulated. In contrast to the limited tissue distribution of SULT2A1, SULT2B1 was detected in a variety of hormone-responsive tissues including placenta, ovary, uterus, and prostate. The catalytic activity toward dehydroepiandrosterone and dihydrotestosterone, biologically important androgens, coupled with expression in prostate suggests that SULT2B1 may play a novel regulatory role that protects against the mitogenic effects of androgens.

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^☆: Abbreviations used: SULT, sulfotransferase; Hsa, Homo sapians; Cpo, Cavia porcellus; Mmu, Mus musculus; DHEA, dehydroepiandrosterone; DHT, dihydrotestosterone.

¹: To whom correspondence should be addressed. Fax: (215) 728-4333. E-mail: [email protected].

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Regular ArticleBiochemical Characterization and Tissue Distribution of Human SULT2B1☆

Abstract

Chem.-Biol. Interact.

Genomics.

Biochem. Biophys. Res. Commun.

Genomics

J. Steroid Biochem. Mol. Biol.

Biochem. Biophys. Res. Commun.

Biochem. Pharmacol.

Genomics

J. Biol. Chem.

Arch. Biochem. Biophys.

J. Biol. Chem.

J Biol Chem.

FEBS Lett.

Sulfation and sulfotransferases 1: Sulfotransferase molecular biology: cDNAs and genes

FASEB J.

Enzymology of human cytosolic sulfotransferases

FASEB J.

Regular Article
Biochemical Characterization and Tissue Distribution of Human SULT2B1☆