Elsevier

Genomics

Volume 68, Issue 2, 1 September 2000, Pages 161-166
Genomics

Regular Article
A Novel Human Cytochrome P450 4F Isoform (CYP4F11): cDNA Cloning, Expression, and Genomic Structural Characterization,☆☆

https://doi.org/10.1006/geno.2000.6276Get rights and content

Abstract

By a combination of cDNA library screening and rapid amplification of cDNA ends analysis, a novel human cytochrome P450 4F isoform has been cloned and sequenced. The new 4F isoform is designated CYP4F11 and contains 1765 nucleotides. The coding region encodes 524 amino acid residues, and the heme-binding region is highly conserved. The CYP4F11 amino acid sequence has 80.0, 82.3, and 79.2% identity to CYP4F2, CYP4F3, and CYP4F8 amino acid sequences, respectively. In vitro translation shows the molecular mass of CYP4F11 is approximately 57 kDa, consistent with the calculated molecular mass. CYP4F11 is expressed mainly in human liver, followed by kidney, heart, and skeletal muscle. The genomic structure of CYP4F11 was solved by database searching and computer analysis. The coding region of CYP4F11 has 12 exons. The CYP4F11 gene is located 16 kb upstream of the CYP4F2 gene on chromosome 19. This is consistent with the notion that the human cytochrome P450 4F genes form a cluster on chromosome 19.

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The data presented here form part of the dissertation of Xiaoming Cui submitted to the faculty of The University of Texas Health Science Center at Houston, Graduate School of Biomedical Sciences, in partial fulfillment of the requirements for the Doctor of Philosophy degree.

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Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under Accession No. AF236085.

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