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HIV Protocols pp 375–391Cite as

Multiparameter Flow Cytometry Monitoring of T Cell Responses

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Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 485))

Abstract

HIV vaccine research increasingly uses polychromatic flow cytometry as a tool to monitor T cell responses. The use of this technology allows for the analysis of highly defined subsets of cells with unique phenotypes and functions. Ultimately, such studies may identify surrogate markers of protection from disease progression. However, this powerful technology comes with a number of technical hurdles, and there is a need to standardize the assays and protocols used in clinical trial monitoring. Here an optimized protocol, with variations for specific circumstances, is presented. This protocol covers the analysis of multiple cytokines, cell surface markers, and other functional markers such as perforin, CD107, and CD154. While the protocol can be adapted to various numbers of fluorescence parameters, optimized panels of 8–10 colors are presented.

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Acknowledgements

Details of this protocol were optimized by Laurel Nomura and Maria Suni (BD Biosciences).

Author information

Authors and Affiliations

  1. Palo Alto, CA, USA

    Holden T. Maecker

Authors
  1. Holden T. Maecker
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Editor information

Editors and Affiliations

  1. Albert Einstein College of Medicine, Bronx, NY, USA

    Vinayaka R. Prasad PhD  & Ganjam V. Kalpana PhD  & 

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Cite this protocol

Maecker, H.T. (2009). Multiparameter Flow Cytometry Monitoring of T Cell Responses. In: Prasad, V.R., Kalpana, G.V. (eds) HIV Protocols. Methods In Molecular Biology™, vol 485. Humana Press. https://doi.org/10.1007/978-1-59745-170-3_25

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  • DOI: https://doi.org/10.1007/978-1-59745-170-3_25

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-859-1

  • Online ISBN: 978-1-59745-170-3

  • eBook Packages: Springer Protocols

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