Abstract
Glutathione S-transferase (GST) was investigated with benzo(a)pyrene-4,5-oxide (BPO) as substrate in tissue specimens from 26 fetal and 27 adult livers and 27 placentas. The average (±SEM) of GST activity in the cytosol was 1.80±0.18 (fetal liver), 3.05 ± 0.30 (adult liver) and 1.18 ±0.07 (placenta) nmol/min/mg. GST was also investigated in human fetal and adult lungs, kidneys and gut. In these tissues the average (±SEM) GST activity ranged between 0.71±0.12 (adult intestine) and 2.11±0.18 (fetal lungs) nmol/min/mg. Whereas in the fetal liver the conjugation of BPO was catalyzed at a rate of about two-thirds of the adult rate, similar or higher GST activities were found in the fetal non-hepatic tissues as compared to the adult organs. No correlation was found between the activity of the GST in fetal liver and placenta and the gestational age (11–25 weeks). GST develops before the 11th week of gestation and it does not undergo changes during the mid-gestation. No correlation was found between GST activity in adult liver and age (32–70 years).
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References
Baars AL, Mukhtar H, Zoetemelk CEM, Jansen M, Breimer D (1981) Glutathione S-transferase activity in rat and human tissues and organs. Comp Biochem Physiol 70: 285–288
Fryer AA, Hume R, Strange RC (1986) The development of glutathione S-transferase and glutathione peroxidase activities in human lung. Biochim Biophys Acta (in press)
Guthenberg C, Mannervik B (1981) Glutathione S-transferase (transferase) from human placenta is identical or closely related to glutathione S-transferase (transferase) from erytrocytes. Biochim Biophys Acta 661: 255–260
Hales BB, Neims AH (1976) Developmental aspects of glutathione S-transferase B (ligandin) in rat liver. Biochem J 160: 231–236
James MO, Foureman GL, Law FC, Bend JR (1977) The perinatal development of epoxide-metabolizing enzyme activities in liver and extrahepatic organs of guinea pig and rabbit. Drug Metab Dispos 5: 19–28
Kamisaka K, Habig WH, Ketley JN, Arias IM, Jacoby WB (1975) Multiple forms of human glutathione S-transferase and their affinity for bilirubin. Eur J Biochem 60: 153–161
Koskelo K, Icen A (1984) Chromatofocusing of gluthatione S-transferases from human kidney. Scand J Clin Lab Invest 44: 159–162
Koskelo K, Valmet E, Tenhuen R (1981) Purification and characterization of an acid glutathione S-transferase from human lung. Scand J Clin Lab Invest 41: 683–689
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275
Mannervik B (1975) The isoenzymes of glutathione transferase. Adv Enzymol 57: 357–417
McManus ME, Boobis AR, Pacifici GM, Frempong RY, Brodie MJ, Kahn GC, Whyte C, Davies DS (1980) Xenobiotic metabolism in the human lung. Life Sci 26: 481–487
Mukhtar H, Bresnick E (1976) Glutathione-S-epoxide transferase activity during development and the effect of partial hepatectomy. Cancer Res 36: 937–940
Mukhtar H, Bend JR (1977) Serum glutathione S-transferase: perinatal development, sex difference and effect of carbon tetrachloride administration on enzyme activity in the rat. Life Sci 21: 1277–1286
Mukhtar H, Zoetemelk CEM, Baars AJ, Wijnen J Th, Blankenstein-Wijnen LMM, Khan PM, Breimer DD (1981) Glutathione S-transferase activity in human fetal and adult tissues. Pharmacology 22: 322–329
Pacifici GM, Rane A (1981) Glutathione S-epoxidetransferase in the human placenta at different stages of pregnancy. Drug Metab Dispos 9: 472–475
Pacifici GM, Rane A (1982) Metabolism of styrene oxide in different human fetal tissues. Drug Metab Dispos 10: 302–305
Pacifici GM, Boobis AR, Brodie MJ, McManus ME, Davies DS (1981a) Tissue and species differences in enzymes of epoxide metabolism. Xenobiotica 11: 73–79
Pacifici GM, Norlin A, Rane A (1981b) Glutathione S-transferase in human fetal liver. Biochem Pharmacol 24: 3367–3371
Pacifici GM, Davies DS, Whyte C, Boobis AR (1982) Tissue differences in the ontogeny of inducibility of drug-metabolizing enzymes by 3-methylcholanthrene in the rabbit. Xenobiotica 12:591–598
Pacifici GM, Warholm M, Guthenberg C, Mannervik B, Rane A (1986) Organ distribution of glutathione transferase isoenzymes in the human fetus: differences between liver and extrahepatic tissues. Biochem Pharmacol 35: 1616–1619
Partridge CA, Dao DD, Awasthi YC (1984) Glutathione S-transferases of lung: purification and characterization of human lung glutathione S-transferases. Lung 162: 27–36
Polidoro G, Di Ilio C, Del Boccio G, Zulli P, Federici G (1980) Glutathione S-transferase activity in human placenta. Biochem Pharmacol 29: 1677–1680
Ryan AJ, James MO, Ben-Zvi Z, Law FCP, Bend JR (1976) Hepatic and extrahepatic metabolism of 14C-styrene oxide. Environ Health Perspect 17: 135–144
Sherman M, Titmuss S, Kirsch RE (1983) Glutathione S-transferase in human organs. Biochem Int 6: 109–118
Shoemaker DD, Dietrick DD, Cysyk RL (1981) Induction and development of mouse liver glutathione S-transferase activity. Experientia 37: 445–446
Sims P, Grover PL (1974) Epoxides in polycyclic anomalic hydrocarbon metabolism out carcinogenesis. Adv Cancer Res 30: 166–274
Smith BR, Bend JR (1980) A prediction of pulmonary benzo(a)pyrene-4,5-oxide clearance: A pharmacokinetic analysis of epoxide-metabolizing enzymes. J Pharmacol Exp Ther 214: 478–482
Soma Y, Satoh K, Sato K (1986) Purification and subnit-structural and immunological characterization of five glutathione S-transferases in human liver, and the acidic form as a hepatic tumor marker. Biochim Biophys Acta 869: 247–258
Stockman PK, Beckett GJ, Hayes JD (1985) Identification of a basic hybrid glutathione S-transferase from human liver. Biochem J 227: 457–465
Turner JC (1969) Tritium counting with Triton X-100 Scintillant. Int J Appl Radiat Isotopes 20: 499–505
Vander Jagt DL, Hunsaker LA, Garcia KB, Royer RE (1985) Isolation and characterization of the multiple glutathione S-transferases from human liver. J Biol Chem 260: 11603–11610
Warholm M, Guthenberg C, Mannervik B, von Bahr C (1981a) Purification of a new glutathione S-transferase (transferase μ) from human liver having high activity with benzo(a)pyrene-4,5-oxide. Biochem Biophys Res Commun 98: 512–519
Warholm M, Guthenberg C, Mannervik B, Pacifici GM, Rane A (1981b) Glutathione S-transferases in human fetal liver. Acta Chem Scand B35:225–227
Warholm M, Guthenberg C, Mannervik B (1983) Molecular and catalytic properties of glutathione transferase μ from human liver: an enzyme efficiently conjugating epoxides. Biochemestry 22: 3610–3617
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Pacifici, G.M., Franchi, M., Colizzi, C. et al. Glutathione S-transferase in humans: development and tissue distribution. Arch Toxicol 61, 265–269 (1988). https://doi.org/10.1007/BF00364848
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DOI: https://doi.org/10.1007/BF00364848