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Residence time distributions of solutes in the perfused rat liver using a dispersion model of hepatic elimination: 1. Effect of changes in perfusate flow and albumin concentration on sucrose and taurocholate

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Abstract

The residence time distributions of sucrose and taurocholate have been determined from the outflow concentration-time profiles after bolus input into an in situperfused rat liver preparation. The normalized variance (and the dispersion number) appeared to be independent of perfusate flow rate (10 to 37ml/mm) and perfusate albumin concentration (0–5%). The apparent volume of distribution for sucrose appeared to increase with flow rate but was unaffected by the concentration of albumin (0–5%) present in the perfusate. The changes in taurocholate availability with flow rate were adequately accounted for by the dispersion model, whereas taurocholate availabilityprotein binding changes required an albumin-mediated transport model to be used in conjunction with the dispersion model.

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Author notes

  1. Michael S. Roberts

    Present address: Departments of Pharmacy and Medicine, University of Queensland, 4067, St. Lucia, Queensland, Australia

Authors and Affiliations

  1. Department of Pharmacy, Otago Medical School, Dunedin, New Zealand

    Michael S. Roberts

  2. School of Pharmacy, University of Tasmania, Hobart, Australia

    Sharon Fraser & Andrew Wagner

  3. Department of Physiology, University of Tasmania, Hobart, Australia

    Lyndsay McLeod

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  1. Michael S. Roberts
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  2. Sharon Fraser
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  4. Lyndsay McLeod
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Additional information

This study was supported by the National Health and Medical Research Council of Australia and the Dean's MRC (NZ) Fund.

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Roberts, M.S., Fraser, S., Wagner, A. et al. Residence time distributions of solutes in the perfused rat liver using a dispersion model of hepatic elimination: 1. Effect of changes in perfusate flow and albumin concentration on sucrose and taurocholate. Journal of Pharmacokinetics and Biopharmaceutics 18, 209–234 (1990). https://doi.org/10.1007/BF01062200

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