Skip to main content
Log in

Differences in membrane ion transport between hepatocytes from the periportal and the pericentral areas of the liver lobule

Experientia Aims and scope Submit manuscript

Abstract

We studied the Na+/K+ pump, Na+/K+ ATPase activity, and oxygen consumption (QO2) in hepatocytes isolated from the periportal (PH) and pericentral (CH) regions of the liver lobule, to provide an insight into the functional properties of these cells. Na+/K+ pump activity was determined using86Rb+ (a functional analog of K+) and ouabain, a specific inhibitor of this transport system. Our results indicate the the Na+/K+, pump and Na+/K+ ATPase activity are significantly lower in CH than in PH, although basal ouabain-sensitive (OS) QO2 was negligible in both of these cell preparations. However, OSQO2 was significantly lower in CH than in PH when the Na+/K+ pump was activated using the ionophore nystatin in a Na+-containing medium. These results indicate that the differences in membrane ion transport exist between hepatocytes from different locations of the liver lobule.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Loud, A. V., J. Cell Biol.37 (1968) 27.

    Article  PubMed  Google Scholar 

  2. Jungermann, K., and Katz, N., in: Regulation of Hepatic Metabolism, p. 211. Eds. R. G. Thurman, R. C. Kauffman and K. Jungermann. Plenum Press, New York and London 1986.

    Google Scholar 

  3. Thurman, R. G., Ji, S., and Lemasters, J., in: Regulation of Hepatic Metabolism, p. 293. Eds. R. G. Thurman, F. C. Kauffman and K. Jungermann. Plenum Press, New York and London 1986.

    Google Scholar 

  4. Katz, N., in: Regulation of Hepatic Metabolism, p. 237. Eds. R. G. Thurman, F. C. Kauffman and K. Jungermann. Plenum Press, New York and London 1986.

    Google Scholar 

  5. Haussinger, D., and Gerok, W., in: Regulation of Hepatic Metabolism, p. 253. Eds. R. G. Thurman, F. C. Kauffman and K. Jungermann. Plenum Press, New York and London 1986.

    Google Scholar 

  6. Fehlmann, M., and Freychet, P., J. biol. Chem.256 (1981) 7449.

    PubMed  Google Scholar 

  7. Berthon, B., Capiod, T., and Claret, M., Br. J. Pharmacol.86 (1985) 151.

    PubMed  Google Scholar 

  8. Lynch, C. J., Wilson, P. B., Blackmore, P. F., and Eston, J. H., J. biol. Chem.261 (1986) 14551.

    PubMed  Google Scholar 

  9. Quistorff, B., Grunnet, N., and Cornell, N. W., Biochem. J.226 (1985) 289.

    PubMed  Google Scholar 

  10. Quistorff, B., Biochem. J.229 (1987) 221.

    Google Scholar 

  11. Lindo, K., and Penttela, K., Biochem. J.228 (1985) 757.

    PubMed  Google Scholar 

  12. HogeBoom, G., and Schneider, W., J. biol. Chem.204 (1953) 233.

    PubMed  Google Scholar 

  13. Brezis, M., Rosen, S., Silva, P., Spokes, K., and Epstein, F. H., Science224 (1984) 66.

    PubMed  Google Scholar 

  14. Lesko, L., Donlon, M., Marinetti, G. B., and Hare, J. D., Biochim. biophys. Acta311 (1973) 173.

    PubMed  Google Scholar 

  15. Fiske, C. H., and SubbaRow, Y., J. biol. Chem.66 (1925) 375.

    Google Scholar 

  16. Bernstein, J., Videla, L., and Israel, L., Ann. N. Y. Acad. Sci.242 (1974) 560.

    PubMed  Google Scholar 

  17. Escobales, N., and Bernstein, J., Puerto Rico Health Sciences Journal1 (1983) 63.

    Google Scholar 

  18. Clark, D. G., Brinkman, M., Filsell, O. H., Lewis, S. J., and Berry, M. N., Biochem. J.202 (1982) 661.

    PubMed  Google Scholar 

  19. Nobes, C. D., Lain-Thomas, P. L., and Brand, M. D., Biochim. biophys. Acta976 (1989) 241.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sillau, A.H., Escobales, N. & Juarbe, C. Differences in membrane ion transport between hepatocytes from the periportal and the pericentral areas of the liver lobule. Experientia 52, 554–557 (1996). https://doi.org/10.1007/BF01969727

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01969727

Key words

Navigation