Abstract
A considerable amount of information has been obtained about human enzymes involved in drug metabolism. Cytochrome P450 is presented as a paradigm in order to illustrate the experimental techniques now available.In vitro assays can be done with tissue slices, microsomes, or even short-term cell cultures. In addition, recombinant enzymes can be produced in a variety of different vector systems. Information about catalytic selectivity regarding new drugs can be obtained, as well as important information about potential drug-drug interactions and bioavailability. Such studies play a role in the drug development process as well as metabolism and safety assessment.
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Guengerich, F.P. In Vitro techniques for studying drug metabolism. Journal of Pharmacokinetics and Biopharmaceutics 24, 521–533 (1996). https://doi.org/10.1007/BF02353478
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DOI: https://doi.org/10.1007/BF02353478