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Identification of IY81149 and its metabolites in the rat plasma using the on-line HPLC/ESI mass spectrometry

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Abstract

Reversed-phase high-performance liquid chromatography/mass spectrometry (HPLC/MS) with an electrospray ionization (ESI) interface was applied to the identification of metabolites of IY 81149 in the rat plasma. Fragments obtained using collision-induced dissociation (CID) in both positive and negative modes were utilized to elucidate the structure of metabolites. The eluent from the conventional HPLC column was split and directly introduced into an ESI-mass spectrometer for the identification of the structures. The CID technique allowed the sensitive identification of sulfonyl-IY81149 and hydroxy-IY81149 from the rat plasma.

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Authors and Affiliations

  1. Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, Cheongryang, P.O. Box 131, 130-650, Seoul, Korea

    Seung-Woon Myung, Hye-Ki Min, Changbae Jin & Myungsoo Kim

  2. Central Research Laboratory, IL YANG Pharmaceutical Company, LTD., 359 Bora-Ri, Kiheung-Eup, Yongin-Si, Kyunggi-Do, Korea

    Seung Mok Lee, Gi Ju Chung, Seong Jun Park & Dong Yeon Kim

  3. Department of Chemistry, KyunGi University, 442-760, Suwon, Kyonggi-do, Korea

    Hyun-Woo Cho

Authors
  1. Seung-Woon Myung
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  2. Hye-Ki Min
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  3. Changbae Jin
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  4. Myungsoo Kim
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  5. Seung Mok Lee
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  6. Gi Ju Chung
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  7. Seong Jun Park
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  8. Dong Yeon Kim
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  9. Hyun-Woo Cho
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Correspondence to Seung-Woon Myung.

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Myung, SW., Min, HK., Jin, C. et al. Identification of IY81149 and its metabolites in the rat plasma using the on-line HPLC/ESI mass spectrometry. Arch Pharm Res 22, 189–193 (1999). https://doi.org/10.1007/BF02976545

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  • DOI: https://doi.org/10.1007/BF02976545

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