Abstract
Nerve growth factor (NGF) exerts various actions on neuronal and non-neuronal tissues and has potential therapeutic utility, but difficulties in using the whole protein have stimulated interest in small NGF fragments. We radioiodinated a small cyclic peptide derived from NGF using the Bolton-Hunter method [125I-C(92-96)], and confirmed binding to high affinity NGF receptors by cross-linkage analysis. Pharmacokinetic characteristics in intravenously injected mice were T 1/2 α 5.2 min, T1/2 β 121.3 min, clearance 11.8 ± 0.5 m//min, and volume of distribution 69.7 ± 4.6ml. Dose-proportionate increases in areas-under-curve and peak-concentrations indicated linear pharmacokinetics. Biodistribution data revealed that clinically relevant doses allowed C(92-96) accumulation sufficient to elicit biological responses in receptor expressing organs including the lungs, liver, spleen, and pancreas.
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Jung, KH., Kim, DH., Paik, JY. et al. Pharmacokinetics and biodistribution of a small radioiodine labeled nerve growth factor fragment. Ann Nucl Med 20, 535–540 (2006). https://doi.org/10.1007/BF03026817
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DOI: https://doi.org/10.1007/BF03026817