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Single nucleotide polymorphisms of ABCB1 (MDR1) gene and distinct haplotype profile in a West Black African population

  • Pharmacogenetics
  • Published:
European Journal of Clinical Pharmacology Aims and scope Submit manuscript

Abstract

Objective

The ABCB1 (MDR1) multidrug transporter plays a key role in determining drug bioavailability. Differences in drug response exist among different ethnic groups. However, until now, no haplotype data are available in a Black African population.

Methods

Exons 2, 7, 10, 11, 12, 14, 17, 21, 26, and the surrounding intronic regions were sequenced using genomic DNA from 111 Beninese subjects to examine 19 intragenic single nucleotide polymorphisms (SNPs). Linkage disequilibrium analysis and haplotypes were generated using the expectation–maximization algorithm.

Results

We identified 12 SNPs, 3 of which were novel: IVS9-57delA, IVS9-8T>A, 1662G>C (exon 14). The most common SNP was IVS14+38A>G. At the MRD1 locus, 53 haplotypes were inferred from the SNP data sets. The 4 SNPs, IVS6+139C>T, IVS9-44A>G, 1236C>T, and 3435C>T, showed strong linkage disequilibrium with each other, confirming the block concept. Moreover, our findings suggest that ABCB1 exonic SNPs are less frequently observed in our population than in African-Americans.

Conclusion

Our data are compatible with a close evolutionary relationship in Black Africans from Benin.

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Acknowledgements

We would like to thank Michel Heusterspreute and Vital Ekanmian for their helpful technical assistance. This study was supported by the Islamic Development Bank and Walloon Region (Waleo 2002, Grant n. 215131/0938840). None of the authors claims any conflict of interest.

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Authors and Affiliations

  1. Centre d’Investigations Cliniques et de Recherches Thérapeutiques, Covè, Benin

    Aurel C. Allabi

  2. Clinical Pharmacology Unit, St Luc University Hospital (UCL), Brussels, Belgium

    Aurel C. Allabi & Yves Horsmans

  3. Laboratory of Applied Molecular Technology Center for Human Genetics, St Luc University Hospital (UCL), Clos Chapelle-aux-Champs, 30-UCL/30.46, 1200, Brussels, Belgium

    Aurel C. Allabi, Bouchra Issaoui & Jean-Luc Gala

  4. Defence Laboratories Department (DG MR), Brussels, Belgian Armed Forces

    Jean-Luc Gala

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  1. Aurel C. Allabi
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  2. Yves Horsmans
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  4. Jean-Luc Gala
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Correspondence to Jean-Luc Gala.

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Allabi, A.C., Horsmans, Y., Issaoui, B. et al. Single nucleotide polymorphisms of ABCB1 (MDR1) gene and distinct haplotype profile in a West Black African population. Eur J Clin Pharmacol 61, 97–102 (2005). https://doi.org/10.1007/s00228-004-0879-0

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  • DOI: https://doi.org/10.1007/s00228-004-0879-0

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