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A Specific Picomolar Hybridization-Based ELISA Assay for the Determination of Phosphorothioate Oligonucleotides in Plasma and Cellular Matrices

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Purpose

To develop and validate an ultrasensitive and specific hybridization-based enzyme-linked immunosorbent assay method for quantification of two phosphorothioate oligonucleotides (PS ODNs) (G3139 and GTI-2040) in biological fluids.

Methods

This assay was based on hybridization of analytes to the biotin-labeled capture ODNs followed by ligation with digoxigenin-labeled detection ODN. The bound duplex was then detected by anti-digoxigenin-alkaline phosphatase using Attophos® (Promega, Madison, WI, USA) as substrate. S1 nuclease and major factors such as the hybridization temperature, concentration of capture probe, and the use of detergent were evaluated toward assay sensitivity, selectivity, and accuracy.

Results

The method is selective to the parent drugs with minimal cross-reactivity (<6%) with 3′-end deletion oligomers for both G3139 and GTI-2040. A linear range of 0.05 to 10 nM (r2 > 0.99) was observed for GTI-2040 in a variety of biological matrices. For both G3139 and GTI-2040, the within-day precision and accuracy values were found to be <20% and 90–110%, respectively; the between-day precision and accuracy were determined to be <20% and 90–120%. Addition of S1 nuclease combined with washing step greatly improved the assay linearity and selectivity. The utility of this assay was demonstrated by simultaneous determination of GTI-2040 in plasma and its intracellular levels in treated acute myeloid leukemia patients.

Conclusions

The validated hybridization enzyme-linked immunosorbent assay method is specific for quantitation of PS ODNs in biological samples to picomolar level. This method provides a powerful technique to evaluate plasma pharmacokinetics and intracellular uptake of PS ODNs in patients and shows its utility in clinical evaluations.

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Abbreviations

AML:

acute myeloid leukemia

AP:

alkaline phosphatase

AS ODN:

antisense oligonucleotide

AUC:

area under the curve

BM:

bone marrow

CL:

total body clearance

C ss :

steady state concentration

CV:

coefficient of variation

Dig:

digoxigenin

ELISA:

enzyme-linked immunosorbent assay

LLOQ:

lower limit of quantification

LOD:

limit of detection

mRNA:

messenger ribonucleic acid

PBMC:

peripheral blood mononuclear cell

PD:

pharmacodynamics

PK:

pharmacokinetics

PS ODN:

phosphorothioate oligonucleotide

RBC:

red blood cells

RNase H:

ribonuclease H

RNR:

ribonucleotide reductase

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Acknowledgments

We acknowledge the support by NIH R21CA105879 and UO1 CA 76576.

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Correspondence to Kenneth K. Chan.

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Wei, X., Dai, G., Marcucci, G. et al. A Specific Picomolar Hybridization-Based ELISA Assay for the Determination of Phosphorothioate Oligonucleotides in Plasma and Cellular Matrices. Pharm Res 23, 1251–1264 (2006). https://doi.org/10.1007/s11095-006-0082-3

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  • DOI: https://doi.org/10.1007/s11095-006-0082-3

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