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Higher Serum DPP-4 Enzyme Activity and Decreased Lymphocyte CD26 Expression in Type 1 Diabetes

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Pathology & Oncology Research

Abstract

Dipeptidyl peptidase-4 (DPP-4) is involved in the metabolism of peptide hormones, T-cell activation and proliferation. In type 1 diabetes mellitus (T1DM) β-cell destruction involves a number of dysregulated T-cells. Our aim was to assess the serum DPP-4 activity and the lymphocyte membrane bound CD26 expression in patients with type 1 diabetes and healthy controls. Ninety-eight (T1DM: 48, F/M = 20/28, mean age: 34.4y; control: 50, F/M = 39/11 mean age: 32,4y) individuals were included. Fasting serum DPP-4 enzymatic activity, plasma glucose (FPG), CD26 expression on CD3+, CD4+ and CD8+ lymphocytes, HbA1c and body mass index (BMI) were assessed. ICA and GAD antibodies were assessed in the T1DM group. DPP-4 enzymatic activity was determined by kinetic enzyme assay, ICA and GAD were assessed by ELISA. Determination of the CD26 expression on CD3+, CD4+ and CD8+ lymphocytes was performed by flow-cytometric analysis. We found higher serum DPP-4 activity (Mean: T1DM: 30.069 U/L, control: 22.62 U/L, p < 0.0001) and decreased CD26 lymphocyte expression on all lymphocyte subpopulations in T1DM. Fasting serum DPP-4 activity was independent from the ICA or GAD status of patients with T1DM. Here we first present that the serum DPP-4 activity is increased and the lymphocyte membrane bound CD26 expression is decreased in type 1 diabetes. Decreased lymphocyte membrane bound CD26 expression in T1DM might be a novel part of the T-lymphocyte regulatory dysfunction observed in type 1 diabetes mellitus. These results might provide some basis for the clinical implication of DPP-4 inhibition in patients with T1DM.

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Abbreviations

DPP-4 CD26:

Dipeptidyl peptidase-4

ICA:

Islet cell antibody

GAD:

Glutamic acid decarboxylase antibody

GLP-1:

Glucagon like peptide-1

HbA1C:

Glycated haemoglobin

BMI:

Body mass index

FPG:

Fasting plasma glucose

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Acknowledgments

This research was supported by the 258/2009 ETT, the PD-73606 OTKA grants and the Hungarian Diabetes Association.

Competing interests

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

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Authors and Affiliations

  1. 2nd Department of Internal Medicine, Semmelweis University, Budapest, Hungary

    Tímea Varga, Anikó Somogyi, Barna Wichmann, Géza Nagy, Károly Rácz & Gábor Firneisz

  2. 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary

    Gábor Barna

  3. Department of Vascular Surgery, Semmelweis University, Budapest, Hungary

    László Selmeci

Authors
  1. Tímea Varga
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  2. Anikó Somogyi
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  3. Gábor Barna
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  4. Barna Wichmann
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  5. Géza Nagy
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  6. Károly Rácz
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  7. László Selmeci
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  8. Gábor Firneisz
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Correspondence to Tímea Varga, Anikó Somogyi or Gábor Firneisz.

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Varga, T., Somogyi, A., Barna, G. et al. Higher Serum DPP-4 Enzyme Activity and Decreased Lymphocyte CD26 Expression in Type 1 Diabetes. Pathol. Oncol. Res. 17, 925–930 (2011). https://doi.org/10.1007/s12253-011-9404-9

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  • DOI: https://doi.org/10.1007/s12253-011-9404-9

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