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Determination of lipid phosphorus in the nanomolar range

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References (1)

  • K. Itaya et al.

    Clin. Chim. Acta

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    The filtrate was dissolved in 0.1 ml of a mixture of methanol/water (95:5, v/v) containing 5 mM ammonium formate for LC-MS/MS. In case of NAPE analysis, lipids were dissolved in the solvent without ammonium formate. The lipid phosphorus concentration in the lipid extract was determined as described previously [28]. LC-MS/MS was performed using a quadrupole-linear ion trap hybrid mass spectrometer, 4000 Q TRAP (Applied Biosystems/MDS Sciex, Concord, Ontario, Canada) with an Agilent 1100 liquid chromatograph (Agilent Technologies, Wilmington DE, USA) and HTSPAL autosampler (CTC Analytics AC, Zwingen, Switzerland), as described previously [17,24,29].

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