Short communicationA linear standard curve for the Folin Lowry determination of protein
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2020, Bioresource TechnologyCitation Excerpt :The supernatants were filtered by polytetrafluoroethylene membranes of 0.45 μm pore size before organic analysis. EPS constituents are shown in Table 1, protein and humic acid contents were determined by the Lowry procedure using bovine serum albumin (BSA) (Sigma) and humic acid (Sigma) at 750 nm and 735 nm by a Hitachi U-3900 h UV/VIS spectrophotometer as described by Stauffer (1975), respectively. And carbohydrate content was determined using anthrone–sulfuric acid colorimetry (Laurentin and Edwards 2003).
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2019, Science of the Total EnvironmentCitation Excerpt :The polysaccharide content in EPS was determined by the phenolsulfuric acid method with glucose as the standard. The protein concentration of EPS was measured by the Lowry procedure with bovine serum albumin (Sigma) and humic acid (Sigma) as described by Stauffer (1975). Three-dimensional excitation emission matrix (3D-EEM) was measured by a Hitachi F-4500 fluorescence spectrophotometer (Esparza-Soto and Westerhoff, 2001).
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2019, Journal of Environmental Sciences (China)Citation Excerpt :The polysaccharide content in EPS was determined by the phenol sulfuric acid method with glucose as the standard. The protein content of EPS was determined by the Lowry procedure with bovine serum albumin (Sigma) and humic acid (Sigma) as described by Stauffer (1975). Three-dimensional excitation emission matrix (3D-EEM) spectra were measured with an F-4500 fluorescence spectrophotometer (Hitachi, Japan) with an excitation range of 200–400 nm at 10 nm sampling intervals and an emission range of 280–500 nm at 10 nm sampling intervals.