A high-yield preparative method for isolation of rat liver mitochondria
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Cited by (175)
A study on the interaction of the amyloid fibrils of α-synuclein and hen egg white lysozyme with biological membranes
2022, Biochimica et Biophysica Acta - BiomembranesCitation Excerpt :Mitochondria from male rat brains were prepared in a pre-cooled isolation buffer A (10 mM Tris-HCl, 1 mM EDTA, 0.32 M sucrose, and 1% BSA (w/v), pH 7.4) using Percoll density gradient centrifugation according to the protocol described by Sims and Anderson [72], with some modifications as described previously [73]. Rat liver mitochondria were isolated in a pre-cooled isolation buffer B (10 mM Tris–HCl, 1 mM EDTA, 0.32 M sucrose, 0.1% BSA (w/v), and 0.25 mM PMSF, pH 7.4) by differential centrifugation using a slightly modified protocol of Bustamante et al. [74]. All centrifugation steps were carried out at 4 °C and mitochondrial membrane integrity was confirmed by measuring citrate synthase activity in isolated mitochondria before and after membrane disruption by Triton X-100.
Dose-dependent regulation of mitochondrial function and cell death pathway by sorafenib in liver cancer cells
2020, Biochemical PharmacologyCitation Excerpt :The animals were sacrificed under general anesthesia by exsanguination from the aortic bifurcation. Liver mitochondria were isolated by differential centrifugation as described by Bustamante et al. [33] with minor modifications. The cut and washed tissue was homogenized by a Teflonglass homogenizer in an ice cold isolation medium containing 220 mM D-mannitol, 70 mM sucrose, 2 mM HEPES, 0.2 mM EGTA, and 0.5 g/l of bovine serum albumin (BSA) fatty acid free, at a pH of 7.2.
Mitochondrial membrane permeabilization upon interaction with lysozyme fibrillation products: Role of mitochondrial heterogeneity
2012, Biochimica et Biophysica Acta - BiomembranesCitation Excerpt :Mitochondria from male rat brains were prepared by differential centrifugation as described earlier [21]. Rat liver mitochondria were isolated in a medium containing [10 mM Tris–HCl (pH 7.4), 1 mM ethylenediaminetetraacetic acid (EDTA), 0.1% (w/v) bovine serum albumin (BSA), 0.32 M sucrose and 0.25 mM phenylmethylsulfonyl fluoride (PMSF)], by differential centrifugation of liver homogenates, using a slightly modified protocol of Bustamante et al. [76]. Briefly, livers from male rat were excised, washed and homogenized in 2 volumes of pre-cooled isolation buffer.
High efficiency of ROS production by glycerophosphate dehydrogenase in mammalian mitochondria
2009, Archives of Biochemistry and BiophysicsCitation Excerpt :Mitochondrial proteins were determined by the Bradford method [28] using BSA as standard. Liver mitochondria were isolated by differential centrifugation essentially as described before [29] in mannitol-based medium (220 mM d-mannitol, 70 mM sucrose, 2 mM HEPES, 0.5 mg.mL−1 BSA, pH 7.2). Tissue homogenate was centrifuged 4 min at 830g and supernatant was centrifuged 15 min at 5190g.
Priming dose of phenylhydrazine protects against hemolytic and lethal effects of 2-butoxyethanol
2007, Toxicology and Applied Pharmacology