Distinct long chain and very long chain fatty acyl CoA synthetases in rat liver peroxisomes and microsomes

doi:10.1016/0003-9861(88)90281-0

Archives of Biochemistry and Biophysics

Volume 266, Issue 2, 1 November 1988, Pages 486-495

https://doi.org/10.1016/0003-9861(88)90281-0 Get rights and content

Abstract

Mitochondria, peroxisomes, and microsomes were isolated from rat liver homogenates, and stearic acid and lignoceric acid β-oxidation, as well as stearoyl CoA synthetase and lignoceroyl CoA synthetase activities in the three organelles, were compared. Stearic acid β-oxidation in peroxisomes was sixfold greater compared to the oxidation in mitochondria. Lignoceric acid β-oxidation, observed only in peroxisomes, was fivefold lower compared to stearic acid β-oxidation. Stearoyl CoA synthetase was present whereas lignoceroyl CoA synthetase was absent in mitochondria. Stearoyl CoA synthetase and lignoceroyl CoA synthetase activities were present in microsomes and peroxisomes, but the activity of stearoyl CoA synthetase was several-fold greater compared to lignoceroyl CoA synthetase in both organelles. The differing responses to detergents and phospholipids of stearoyl CoA and lignoceroyl CoA synthetase activities in microsomes as well as peroxisomes indicated that each activity was catalyzed by a separate enzyme. Differences in detergent and phospholipid response were also noted when either stearoyl CoA or lignoceroyl CoA synthetase activity in one organelle was compared with the corresponding activity in the other organelle, suggesting that the same activity in different organelles may be catalyzed by separate enzyme proteins.

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Distinct long chain and very long chain fatty acyl CoA synthetases in rat liver peroxisomes and microsomes

Abstract

J. Biol. Chem

Biochem. Biophys. Res. Commun

J. Biol. Chem

J. Biol. Chem

Arch. Biochem. Biophys

Arch. Biochem. Biophys

Arch. Biochem. Biophys

Arch. Biochem. Biophys

Clin. Chim. Acta

Clin. Chim. Acta

J. Biol. Chem

Chem. Phys. Lipids

Anal. Biochem

Biochem. Biophys. Res. Commun