Brain cytochrome P450 and testosterone metabolism by rat brain subcellular fractions: Presence of cytochrome P450 3A immunoreactive protein in rat brain mitochondria
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2021, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life SciencesCitation Excerpt :Therefore, they act as a major determinant of the oral bioavailability and systemic clearance of drugs that are metabolized by CYP3A. In addition to the liver and intestine, CYP3A enzymes are also expressed in different areas of the brain at the mRNA, protein, and functional level [10,11]. Additionally, metabolism of CYP3A substrates, like testosterone, erythromycin, and alprazolam, by rat and human brain microsomes has been reported [11–14].
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Melatonin and steroid hormones activate intermembrane Cu,Zn-superoxide dismutase by means of mitochondrial cytochrome P450
2011, Free Radical Biology and MedicineCitation Excerpt :From a physiological perspective, it has been suggested that melatonin may accumulate in some cellular structures such as cell membrane, cytosol, and nuclei; intramitochondrial melatonin levels are 100-fold higher than 1 nM nocturnal plasma concentration [36]. It has long been known that the P450 subfamily is expressed in rat liver microsomes and brain mitochondria [37,38]. Interestingly, it has been recently reported that melatonin metabolism by P450 in vivo is carried out more efficiently in mitochondria than in rat liver microsomes [39].
Hazard evaluation of diisononyl phthalate and diisodecyl phthalate in a Japanese medaka multigenerational assay
2006, Ecotoxicology and Environmental SafetyDetermination of testosterone and its metabolites using liquid chromatography with elevated column temperature and flow-rate gradient
2002, Analytica Chimica ActaCitation Excerpt :The amounts of the metabolites produced by the rat liver microsome after 5 and 15 min of incubation are summarized in Table 3. Rat liver microsome contains cytochrome P-450 enzymes that are known to metabolize testosterone [12,32,33]. We demonstrate the determination of five common testosterone metabolites after testosterone is incubated with rat liver microsome for as short as 5–15 min (Fig. 4).
Rapid high-performance liquid chromatographic method for the separation of hydroxylated testosterone metabolites
2001, Journal of Chromatography B: Biomedical Sciences and Applications