Biochimica et Biophysica Acta (BBA) - Protein Structure
Enzymatic iodination of polypeptides with 125I to high specific activity
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2016, TheriogenologyCitation Excerpt :The primary antibody was rabbit polyclonal antiserum raised against boPAG2 (AS#438) according to the method of Vaitukaitis [36]. Owing to the instability of the boPAG2 molecule, boPAG1 (67 kDa) was used as a standard (dilutions of 100–0.2 ng/mL) and for iodination with the 125I isotope [39]. The initial dilution of the primary AS#438 was 1:2500.
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