Enzymatic iodination of polypeptides with 125I to high specific activity

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Abstract

  • 1.

    1. Lactoperoxidase was extracted from cow milk by a simplified method starting with batch-wise adsorption onto GM-Sephadex-50. It was then purified by (NH4)2SO4 precipitation and isoelectric focusing. This product had an A412nm/A280nm ratio of 0.8–0.9.

  • 2.

    2. Lactoperoxidase together with H2O2 could oxidize carrier-free Na125I to “active iodine” with efficiency to iodinate proteins to high specific activity.

  • 3.

    3. Polypeptide hormones radioiodinated by this technique retained their immunological reactivity and were used in radioimmunoassays with good results.

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