Age and gender-related gene expression of hydroxysteroid sulfotransferase-a in rat liver

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Abstract

Hepatic hydroxysteroid sulfotransferase-a (HST-a) gene expression was examined in young male (age 22–26 days) and female rats (age 22–30 days), and in older male (age 42–45 days) and female (age 49–55 days) rats. Northern and slot blot analyses of poly(A)+RNA revealed that HST-a was differentially expressed with respect to both age and gender with female rats expressing higher levels of HST-a in both age groups. Hepatic HST-a mRNA levels were ≈4 to 6-fold higher in females compared to males in both age groups examined. HST-a expression increased with age in both male and female rats. HST-a expression was ≈8 to 10-fold higher in 42–45 day old males relative to 22–26 day old males. HST-a mRNA levels were ≈3 to 7-fold higher in 49–55 day old females relative to females in the 22–30 day age group. These data suggest that HST-a gene expression is transcriptionally controlled and that HST-a regulation is subject to hormonal and developmental modulation.

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