cDNA cloning and structure of mouse putative Ah receptor

doi:10.1016/0006-291X(92)91185-S

Biochemical and Biophysical Research Communications

Volume 184, Issue 1, 15 April 1992, Pages 246-253

https://doi.org/10.1016/0006-291X(92)91185-S Get rights and content

Abstract

Mouse cDNA clones for a putative Ah receptor have been isolated from a cDNA library of mRNA from Hepa-1 cells by an oligonucleotide probe produced by PCR with a pair of primers which was synthesized according to the reported N-terminal sequence of 26 amino acids. The cDNA clones encode a polypeptide of 805 amino acids with a helix-loop-helix motif and with some similarity to a certain region designated PAS of Drosophila Per and Sim, and human Arnt protein. Cotransfection of an expression vector of the Ah receptor with a reporter plasmid pMC6.3k consisting of CYP1A1 promoter and CAT structural gene into CV-1 cells enhanced the CAT expression in response to added 3-methylcholanthrene.

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Citation Excerpt :
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cDNA cloning and structure of mouse putative Ah receptor

Abstract

J. Biol. Chem.

Arch. Biochem. Biophys.

Cell.

Cell.

J. Biol. Chem.

Cell.

J. Biol. Chem.

Ann. Rev. Pharmacol. Toxicol.

Ann. Rev. Pharmacol. Toxicol.

Mol. Pharmacol.

Mol. Pharmacol.

Nucleic Acid Res.

EMBO J.

Mol. Pharmacol.