Elsevier

Biochemical Pharmacology

Volume 21, Issue 24, 15 December 1972, Pages 3249-3256
Biochemical Pharmacology

Kinetic parameters of drug-metabolizing enzymes in Ca2+-sedimented microsomes from rat liver

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Abstract

Kinetic parameters of five substrates of the mixed function oxidase system were determined to ensure that the mixed function oxidases were unaltered by the Ca2+-sedimented microsomes. Using either of the microsomal preparation procedures, no differences were noted in the Vmax and Km for aminopyrine, ethylmorphine and p-nitroanisole demethylation, aniline hydroxylation and hexobarbital oxidase. Also, no differences were seen in the NADPH cytochrome P-450 reductase activities. The Ca2+-sedimentation procedure was further simplified to allow microsomal preparation within 1 hr.

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This work was supported by United States Public Health Service Research Grant GM17021.

Public Health Service Research Career Development Awardee, GMK4-19,601 of the NIH.

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