Research paperStudies on a ketone reductase in human and rat liver and kidney soluble fraction using warfarin as a substrate
References (15)
- et al.
J. Pharm. Sci.
(1972) - et al.
J. biol. Chem.
(1951) - et al.
Biochem. Med.
(1970) - et al.
J. biol. Chem.
(1968) - et al.
J. Pharm. Pharmac.
(1973) - et al.
J. clin. Invest.
(1974) - et al.
J. Pharmac. exp. Ther.
(1970)
Cited by (53)
Carbonyl reduction of warfarin: Identification and characterization of human warfarin reductases
2016, Biochemical PharmacologyCitation Excerpt :The Km for (RR/SS)-warfarin alcohol was similar in both liver fractions, but the microsomes had a higher Vmax. Until now, there have been only two kinetic studies on the in vitro reduction of warfarin in human liver [22,32]. The study by Moreland et al. [32] was performed only with cytosol, using (R)-warfarin as the substrate.
Determination of warfarin alcohols by ultra-high performance liquid chromatography-tandem mass spectrometry: Application to in vitro enzyme kinetic studies
2014, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life SciencesCitation Excerpt :Warfarin undergoes reduction by hepatic reductases, which generate warfarin alcohols of two diastereoisomers, namely RS/SR-warfarin alcohol (alcohol 1), and RR/SS-warfarin alcohol (alcohol 2) [10,11]. Warfarin reduction is catalyzed predominantly in the cytosol, producing warfarin alcohol 1 as the major metabolite [12,13]. Although reduction accounts for up to 20% of warfarin metabolism [11] and produces pharmacologically active alcohol metabolites [14], the effect of altered reductase function on the disposition of warfarin and its alcohol metabolites has not been studied to date.
Comparative metabolism of warfarin in rats and chickens
2011, Poultry ScienceCitation Excerpt :In particular, an increasing incidence of anticoagulant poisoning in birds has been reported (Eason et al., 2002; Erickson and Urban, 2004). The evident causes in birds are divided into 2 categories: primary exposure by direct consumption of bait, and secondary exposure by consumption of poisoned prey (Albert et al., 2010; Redig and Arent, 2008; Murray and Tseng, 2008). In mammals, warfarin is extensively catalyzed to hydroxywarfarin forms in liver microsomes by hydroxylation reactions in phase 1 metabolic pathways using cytochrome P450 (CYP).
Human P450 metabolism of warfarin
1997, Pharmacology and TherapeuticsAmperometric enzyme biosensors for the analysis of drugs and metabolites
1996, Advanced Drug Delivery ReviewsReduction of drug ketones by dihydrodiol dehydrogenases, carbonyl reductase and aldehyde reductase of human liver
1995, Biochemical Pharmacology