Elsevier

Biochemical Pharmacology

Volume 30, Issue 11, 1 June 1981, Pages 1333-1336
Biochemical Pharmacology

Two systems are involved in the sulfobromophthalein uptake by rat liver cells: One is shared with bile salts

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Abstract

It is generally believed that the uptake of sulfobromophthalein by rat liver cells is mediated by a single carrier. Accordingly, kinetic plots obtained using a wide concentration range, failed to give any evidence of heterogeneity in the uptake and gave a Kmof 8.9 ± 2.5 μM and a maximal velocity of 4.2 ± 0.8 nmoles/min/106 cells. Na+-taurocholate inhibited competitively, but only partially, sulfobromophthalein uptake by isolated rat liver cells. The taurocholate sensitive component of sulfobromophthalein uptake followed Michaelis Menten kinetics with a Kmof 0.9 μM and a Vmax of 0.47 nmole/min/106 cells. Taurocholate inhibition reveals an heterogeneity in the uptake of sulfobromophthalein not shown in classical kinetic plots, indicating that two carriers are involved in the uptake of this dye by rat liver cells. The taurocholate sensitive carrier which displays an affinity for sulfobromophthalein ten times higher than that of taurocholate insensitive one, is probably identical to the Na+-independent carrier involved in bile salt uptake.

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