Inductive and repressive effects of rifampicin on rabbit liver microsomal cytochrome P-450

doi:10.1016/0006-2952(84)90694-4

Biochemical Pharmacology

Volume 33, Issue 17, 1 September 1984, Pages 2771-2776

https://doi.org/10.1016/0006-2952(84)90694-4 Get rights and content

Abstract

New Zealand White rabbits were treated with rifampicin at a dose of 50 mg/kg for 4 days. The total amount of microsomal hepatic cytochrome P-450 was not modified in treated, with respect to control, animals. However, further studies involving SDS-PAGE analysis, monooxygenase activity measurements and radial immunodiffusion assays indicated that rifampicin strongly affects the level of two P-450 isoenzymes. An LM₃ form was induced; this form, apparently associated with erythromycine demethylase activity and hydroxylation of progesterone preferentially in position 6β, was shown to be immunologically and functionally different from LM_3a and LM_3b. On the other hand, an LM₄ form typically induced by β-naphthoflavone, was repressed. The concomitant inductive/repressive effect of rifampicine on two cytochrome P-450 isoenzymes makes this drug a very atypical inducer, at least in the rabbit.

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Inductive and repressive effects of rifampicin on rabbit liver microsomal cytochrome P-450

Abstract

Chest

Gastroenterology

Lancet

J. biol. Chem.

J. biol. Chem.

J. biol. Chem.

Meth. Enzymol.

J. biol. Chem.

Archs Biochem. Biophys.

J. biol. Chem.

J. biol. Chem.

J. biol. Chem.

Biochem. Pharmac.

Chemotherapia

Antimicrob. Agents Chemother.