Evidence that the loss of rat liver cytochrome P450 in vitro is not solely associated with the use of collagenase, the loss of cell-cell contacts and/or the absence of an extracellular matrix
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The methylimidazolium ionic liquid M8OI is detectable in human sera and is subject to biliary excretion in perfused human liver
2021, ToxicologyCitation Excerpt :Additionally, donors were often on a range of drugs that are known to induce xenobiotic metabolising enzymes prior to death. It is also well known that changes (primarily a rapid loss) in xenobiotic metabolising enzymes are initiated during perfusion (Padgham and Paine, 1993) and continue to fall during culture, even in intact tissue (Wright and Paine, 1992; Padgham et al., 1993). Accordingly, the ability of perfused tissue and hepatocytes in culture to fully model M8OI metabolism in man is always in question.
Leading-Edge Approaches for In Vitro Hepatotoxicity Evaluation
2018, Comprehensive Toxicology: Third EditionEvaluation of perfluoroalkyl acid activity using primary mouse and human hepatocytes
2013, ToxicologyCitation Excerpt :It is not unexpected to find differences in gene regulation between hepatocytes in monolayer culture and those from intact tissue. Diminished cytochrome P450 enzymatic activity, for example, is a well described response in cultured primary rat hepatocytes (Kocarek et al., 1993; Wright and Paine, 1992). Boess et al. (2003) further reported that, in addition to a marked loss of Cyp2b mRNA expression and enzymatic activity, a general drift in gene expression could be observed across the transcriptome of cultured rat hepatocytes over time.
Liver fibrosis in vitro: Cell culture models and precision-cut liver slices
2007, Toxicology in Vitro