Short communicationModulation of multidrug resistance gene expression in rat hepatocytes maintained under various culture conditions
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Cited by (28)
Efficient transfection of Xenobiotic Responsive Element-biosensor plasmid using diether lipid and phosphatidylcholine liposomes in differentiated HepaRG cells
2017, International Journal of PharmaceuticsCitation Excerpt :The metabolically competent HepaRG hepatocyte-like cells can be isolated from the cholangiocytes, plated at high density and maintained for to 2–3 weeks with a stable expression of DMEs (Corlu and Loyer, 2015), allowing chronic toxicity and genotoxicity studies (Quesnot et al., 2016). The expression of DMEs in hepatocytes is strongly regulated at the transcriptional level by hormonal factors such as growth factors and cytokines (Fardel et al., 1992; Abdel-Razzak et al., 1993; Langouet et al., 1995; Desmots et al., 2002), hypoxia (Legendre et al., 2009), endogenous noxious compounds and xenobiotics (Jetten et al., 2013). An important aspect in the assessment of drug hepatotoxicity is the induction of DME’s expression by xenobiotics to identify activation of metabolic pathways.
Identification of interspecies difference in efflux transporters of hepatocytes from dog, rat, monkey and human
2008, European Journal of Pharmaceutical SciencesInfluence of isolation procedure, extracellular matrix and dexamethasone on the regulation of membrane transporters gene expression in rat hepatocytes
2002, Biochemical PharmacologyCitation Excerpt :The mdr1b gene, which encode the transport protein located at canalicular membrane and which is responsible of the excretion of organic cations, was strongly up-regulated in both culture systems over a 44-hr period whereas mdr1a was remaining constant. These results, which confirmed those from other investigators [6,48–50], indicate that the two rodent mdr1 genes are differentially regulated, and demonstrate that the addition of an ECM could not prevent the up-regulation of the mdr1b gene. The regulation pattern of mrp2 gene follows a bell-shaped profile with a maximum of expression between 20 and 44 hr of culture in both systems and no effect of the ECM was observed.
Pro-inflammatory cytokines tumor necrosis factor α and interleukin-6 and survival factor epidermal growth factor positively regulate the murine GSTA4 enzyme in hepatocytes
2002, Journal of Biological ChemistryCitation Excerpt :An increase in hGSTA1 and A2 by IL-4 in cultured human hepatocytes (46) and a marked decrease in rGSTA2 and M1 mRNA levels by IL-1β in rat hepatocytes (47) have been evidenced. Moreover, rGSTP1 expression is strongly induced by EGF in cultured rat hepatocytes (48). However, this is the first time, to our knowledge, that induction of several GSTs has been evidenced during liver regeneration and that a correlation has been established between this induction and the soluble factors essential for hepatocyte survival and proliferation.
Differential regulation of multidrug resistance-associated protein 2 (MRP2) and cytochromes P450 2B1/2 and 3A1/2 in phenobarbital-treated hepatocytes
2002, Biochemical PharmacologyCitation Excerpt :MRP2 up-regulation by phenobarbital therefore seems to be unrelated to modulation of the differentiation status of the primary liver cells. The fact that the differentiating agent DMSO, which strongly increased expression of albumin in agreement with previous reports [30,41], failed to enhance MRP2 expression in primary hepatocytes also fully supports this conclusion. Another known phenotypic effect related to high concentrations of phenobarbital corresponds to enhancement of cell survival [28,42].