Research paper4-Ipomeanol and 2-aminoanthracene cytotoxicity in cells expressing rabbit cytochrome P450 4B1
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2023, Toxicology LettersNovel insights into oxidation of fatty acids and fatty alcohols by cytochrome P450 monooxygenase CYP4B1
2020, Archives of Biochemistry and BiophysicsCitation Excerpt :The crystal structure of CYP4B1 solved by Hsu et al. [14] suggests a very narrow substrate binding site directing the ω-position of octane close to the heme iron. Nevertheless, the substrate binding site is at least big enough to accept the furan compounds IPO 13 and PK 14 [11] as well as the even bulkier 2-aminoanthracene [48] as substrates. External factors such as the addition of cytochrome b5 can influence fatty acid hydroxylation activity as well: Loughran et al. [49] reported that the turnover of C12 4 by CYP4A7 increased from 28 to 130 min−1 through the addition of cytochrome b5; based on their data the authors suggested that cytochrome b5 in this particular case did not serve as an electron donor for CYP4A7, but rather took a conformational role in such that it docks at the proximal surface of the heme pocket to open the substrate channel for greater access to substrates of varying flexibility, bulk, and chain length [49].
Pulmonary cytochrome P450 enzymes belonging to the CYP4B subfamily from an Australian marsupial, the tammar wallaby (Macropus eugenii)
2011, Comparative Biochemistry and Physiology - C Toxicology and PharmacologyCitation Excerpt :No CYP4B member has been identified in any Australian marsupial species. In experimental animals, CYP4B1 activates certain pro-toxicants and numerous aromatic amines that are responsible for tissue-specific toxicities, including valproic acid, 3-methylindole, 4-ipomeanol, and certain arylamines such as 2-aminofluorene (2-AF), 2-aminoanthracene (2-AA), 2-acetylaminofluorene (2-AAF), and 3-methoxy-4-aminoazobenzene (3-MeO-AAB) (Robertson et al., 1983; Vanderslice et al., 1985; Imaoka and Funae, 1990; Smith et al., 1995; Imaoka et al., 1995; Baer and Rettie, 2006). The CYP4B1 subfamily is also important in the metabolism of various endogenous substrates such as lauric acid and other lipids, as well as in the synthesis of cholesterol and steroids.
Rabbit CYP4B1 engineered for high-level expression in Escherichia coli: Ligand stabilization and processing of the N-terminus and heme prosthetic group
2003, Archives of Biochemistry and BiophysicsGenotyping and site-directed mutagenesis of a cytochrome P450 meander Pro-X-Arg motif critical to CYP4B1 catalysis
2003, Toxicology and Applied PharmacologyThiourea toxicity in mouse C3H/10T1/2 cells expressing human flavin-dependent monooxygenase 3
2002, Biochemical PharmacologyCitation Excerpt :Integrity of the 5′ and 3′ end, as well as orientation, was confirmed by DNA sequencing 250 bp bracketing the 5′ and 3′ ends of the subcloning site. The pMV7:huFMO 3 vector was converted into an RDRV by calcium phosphate transfection into the GP+E-86 cell line as previously described for the cytochromes P450 [16]. GP+E-86 cells were seeded at 5×105 cells/100 mm dish in DMEM+10% FBS.