Elsevier

Clinica Chimica Acta

Volume 31, Issue 1, January 1971, Pages 87-96
Clinica Chimica Acta

Albumin standards and the measurement of serum albumin with bromcresol green

https://doi.org/10.1016/0009-8981(71)90365-2Get rights and content

Abstract

A rapid and reliable method for measuring serum albumin employing bromcresol green is described. The addition of albumin to a solution of bromcresol green in a 0.075 M succinate buffer pH 4.20 results in an increase in absorbance at 628 nm. The absorbance—concentration relationship is linear for samples containing up to 6 g/dl albumin. Bilirubin, moderate lipemia, and salicylate do not interfere with the analysis. The use of a nonionic surfactant (Brij-35) reduces the absorbance of the blank, prevents turbidity and provides linearity. The results by this method agree very well with those obtained by electrophoresis and salt fractionation. The method is simple, it has excellent precision and the reagents are stable.

A protein standard is introduced which can be employed for both the total serum proteins and albumin determinations.

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Present address: Marquette School of Medicine, Department of Pathology, 8700 West Wisconsin Avenue, Milwaukee, Wis. 53226.

∗∗

Present address: Indiana University School of Medicine, Department of Clinical Pathology, 1100 West Michigan Street, Indianapolis, Indiana 46202.

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