Albumin standards and the measurement of serum albumin with bromcresol green
Abstract
A rapid and reliable method for measuring serum albumin employing bromcresol green is described. The addition of albumin to a solution of bromcresol green in a 0.075 M succinate buffer pH 4.20 results in an increase in absorbance at 628 nm. The absorbance—concentration relationship is linear for samples containing up to 6 g/dl albumin. Bilirubin, moderate lipemia, and salicylate do not interfere with the analysis. The use of a nonionic surfactant (Brij-35) reduces the absorbance of the blank, prevents turbidity and provides linearity. The results by this method agree very well with those obtained by electrophoresis and salt fractionation. The method is simple, it has excellent precision and the reagents are stable.
A protein standard is introduced which can be employed for both the total serum proteins and albumin determinations.
References (18)
- J.V. Pastewka et al.
Clin. Chim. Acta
(1965) - J.S. Bracken et al.
Amer. J. Clin. Pathol.
(1953) - J.W. Keyser
Clin. Chem.
(1968) - D.A. Arvan et al.
Clin. Chim. Acta
(1969) - D.D. Rutstein et al.
J. Clin. Invest.
(1954) - F.L. Rodkey
Clin. Chem.
(1965) - R.J. Bartholomew et al.
- O. Hernandez et al.
Clin. Chem.
(1967) - D. Dow et al.
Clin. Chem.
(1969)
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Present address: Marquette School of Medicine, Department of Pathology, 8700 West Wisconsin Avenue, Milwaukee, Wis. 53226.
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Present address: Indiana University School of Medicine, Department of Clinical Pathology, 1100 West Michigan Street, Indianapolis, Indiana 46202.