The hepatotoxicities of endotoxin and ethanol: Comparisons in vitro using the precesion-cut rat liver slice model
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LVV-hemorphin-7 (LVV-H7) plays a role in antinociception in a rat model of alcohol-induced pain disorders
2021, PeptidesCitation Excerpt :ii) Alcohol directly denatures the proteins by disrupting intramolecular hydrogen bonding. Yet, 10 % ethanol (0.5 g/kg) used in this study has been proven to be metabolized within 4 h in rats [56–59]. To avoid the direct effects caused by acute alcohol administration, we chose 4 h after alcohol administration as the blood sampling time.
Catalase increases ethanol oxidation through the purine catabolism in rat liver
2017, Biochemical PharmacologyCitation Excerpt :Rats were anesthetized with sodium pentobarbital and the heart was perfused with cold Krebs buffer to remove the blood from the liver. We used the precision-cut rat liver slice model [10], where the organ was removed and sliced with a chopper (0.35 mm thickness). About 400 mg of liver slices was placed in a 25-mL Erlenmeyer flask containing 5 mL of cold Krebs buffer (pH 7.4), as well as 1 mmol/L of any substrate, to which ethanol was added to a final concentration of 10 mmol/L.
An in vitro method of alcoholic liver injury using precision-cut liver slices from rats
2008, Biochemical PharmacologyCitation Excerpt :Although primary hepatocytes in culture, hepatoma cell lines and WIF-B cells have provided valuable information, they do not always provide the ability to study intracellular interactions and the regulatory factors associated with naturally occurring extracellular matrices, and in some instances they rapidly lose specific liver gene expression [3–9]. Others have used liver slices in the past to attempt to investigate the effects of ethanol on the liver; however, as with isolated cells and cell lines, these slices rapidly lost all normal liver function [16,28–31]. Recently, investigators have developed precision-cut liver slices (PCLS), incubated under high oxygen content, for investigating the effects of various toxins on the liver [10,14,32,33].
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