Butylated hydroxytoluene (BHT)-mediated increases in NAD(P)H-quinone oxidoreductase (QR) in mouse lung: Evidence for the alveolar type II cell as a site of QR activity

doi:10.1016/0041-008X(88)90248-7

Toxicology and Applied Pharmacology

Volume 96, Issue 1, October 1988, Pages 68-74

https://doi.org/10.1016/0041-008X(88)90248-7 Get rights and content

Abstract

Administration of butylated hydroxytoluene (BHT) to inbred $A J$ mice increased lung cytosolic NAD(P)H-quinone oxidoreductase (QR) activity. Increased QR activity was observed using a dose of BHT of 100 mg/kg ip, although maximal increases in activity were observed following BHT 300 mg/kg ip. Increased lung QR activity could be observed as early as 24 hr post-BHT (300 mg/kg ip). Bronchiolar Clara cells and pulmonary macrophages isolated using an elastase digestion procedure from both control and BHT-treated mice exhibited minimal QR activity. The majority of basal and BHT-increased QR activity remained in the undigested lung. As isolation of many cell types to a high degree of purity from mouse lung is not yet feasible, QR activity was measured in established mouse lung cell lines and in cell isolates from rat lung. QR activity was low in a Clara tumor cell line, but higher activity was observed in three alveolar type II cell lines. Type II cell-enriched populations isolated from rat lung exhibited sixfold greater QR activity than the remaining undigested lung. Pulmonary QR activity was significantly greater in rat than in $A J$ or C57 mice. Treatment of cultured Clara or type II cells with BHT in vitro did not increase QR activity.

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^☆: Presented in part at the 27th Annual Meeting of the Society of Toxicology, Dallas, February 15–19, 1988.

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Butylated hydroxytoluene (BHT)-mediated increases in NAD(P)H-quinone oxidoreductase (QR) in mouse lung: Evidence for the alveolar type II cell as a site of QR activity☆

Abstract

Biochim. Biophys. Acta

Toxicology

Toxicol. Appl. Pharmacol.

Arch. Biochem. Biophys.

J. Biol. Chem.

Toxicol. Appl. Pharmacol.

Toxicol. Appl. Pharmacol.

Pathology

Cell Biol. Int. Rep.

Toxicol. Appl. Pharmacol.

Biochem. Pharmacol.

Food Cosmet. Toxicol.

Adv. Enzyme Regul.

J. Biol. Chem.

Lung injury induced by butylated hydroxytoluene

Lab. Invest.

Elevation of extrahepatic glutathione S-transferase and epoxide hydratase activities by 2(3)-tert-butyl-4-hydroxyanisole

Cancer Res.

Increase of NAD(P)H:quinone reductase by dietary antioxidants: Possible role in protection against chemical carcinogenesis and toxicity