Effect of preexposure to dietary benzo[a]pyrene (BP) on the first-pass metabolism of BP by the intestine of toadfish (Opsanus tau): in vivo studies using portal vein-catheterized fish

Abstract

The effect of preexposure of fish to dietary benzo[a]pyrene (BP) on the intestinal metabolism of BP was examined in toadfish (Opsanus tau). The portal veins of toadfish were cannulated following administration of radiolabeled BP to the intestinal lumen. Because these fish lack a lymphatic vessel system, the portal vein is the sole route by which BP and its metabolites enter the circulation. In fish preexposed to dietary BP (10 mg BP/kg food), the radioactivity entering the portal vein was almost entirely (ca. 90%) BP metabolites. In fish fed a laboratory control diet, a smaller percentage (ca. 60%) of the radioactivity entering the portal vein was in the form of BP metabolites. The enhanced efficiency of the intestines of preexposed fish in metabolizing BP appears to be a result of induction of intestinal aryl hydrocarbon hydroxylase (AHH) activity. Intestinal microsomal AHH activities in control and preexposed fish were 0.033 ± 0.032 and 0.320 ± 0.060 nmol·min⁻¹·mg⁻¹, respectively. Gel filtration of portal vein plasma indicated differences in the roles of plasma proteins in transporting BP and BP metabolites. Native BP was associated primarily with the high density lipoproteins, whereas organic-soluble BP metabolites were associated primarily with serum albumin fractions. A large percentage of BP metabolites was recovered as water-soluble conjugates. These studies indicate that in fish, the intestine can be an important organ involved in dietary BP metabolism.